LOCUS       sequence-311896-        1133 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express a fragment of yeast pol1 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Li Z, Hua X, Serra-Cardona A, Xu X, Gan S, Zhou H, Yang WS, Chen CL,
            Xu RM, Zhang Z
  TITLE     DNA polymerase alpha interacts with H3-H4 and facilitates the 
            transfer of parental histones to lagging strands.
  JOURNAL   Sci Adv. 2020 Aug 26;6(35):eabb5820. doi: 10.1126/sciadv.abb5820. 
            eCollection 2020 Aug.
  PUBMED    32923642
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-08-26"; volume: "6(35):eabb5820. doi"; pages: " 
            10.1126/sciadv.abb5820. eCollection 2020 Aug"
COMMENT     Sequence Label: pGST-pol1-N1
FEATURES             Location/Qualifiers
     source          1..1133
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gap             2..14
                     /estimated_length=13
     CDS             56..76
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(560..582)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(727..745)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        813..917
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cnnnnnnnnn nnnncgtttg gnggtggcga ncatcctccn aaaatcgata cgaccgaaaa
       61 cctgtatttt cagggcgcca tggatccggg atcccatgtc ttctaaaagt gaaaaattag
      121 aaaaattgag aaagctgcaa gccgctcgaa atggtacatc catcgatgat tacgaaggtg
      181 acgaatcaga tggtgacaga atatacgacg aaatcgacga aaaagaatac agggccagaa
      241 agcgccagga attgctgcat gacgattttg ttgtagatga cgatggtgta ggctacgtcg
      301 atcgtggtgt cgaagaagat tggagggaag ttgataacag ttcaagtgat gaagacactg
      361 gaaaccttgc tagcaaggat tctaaaagga agaagaatat aaaaagggag aaagaccacc
      421 agatttgagg aattcaaagg cctacgtcga cgagctcaac tagtgcggcc gctttcgaat
      481 ctagagcctg cagtctcgag cggccgcatc gtgactgact gacgatctgc ctcgcgcgtt
      541 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
      601 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
      661 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtataat tcttgaagac
      721 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
      781 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
      841 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
      901 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
      961 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     1021 aagatcagtt ggtgcccnna agtgggttac atcgaactgg atctcaacag cggtaanntc
     1081 cttganngtt ttnnccccna aaaacgtttt ccaatgatga gcacttttna agg
//
