LOCUS       sequence-311899-        1109 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express a fragment of yeast pol1 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Li Z, Hua X, Serra-Cardona A, Xu X, Gan S, Zhou H, Yang WS, Chen CL,
            Xu RM, Zhang Z
  TITLE     DNA polymerase alpha interacts with H3-H4 and facilitates the 
            transfer of parental histones to lagging strands.
  JOURNAL   Sci Adv. 2020 Aug 26;6(35):eabb5820. doi: 10.1126/sciadv.abb5820. 
            eCollection 2020 Aug.
  PUBMED    32923642
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-08-26"; volume: "6(35):eabb5820. doi"; pages: " 
            10.1126/sciadv.abb5820. eCollection 2020 Aug"
COMMENT     Sequence Label: pGST-pol1-N2-2A2
FEATURES             Location/Qualifiers
     source          1..1109
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             42..62
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(741..763)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(908..926)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        994..1098
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgtttgntgg nggcgaccnt cctccnaaaa tcgatacgac cgaaaacctg tattttcagg
       61 gcgccatgga tccgggatcc catgtcttct aaaagtgaaa aattagaaaa attgagaaag
      121 ctgcaagccg ctcgaaatgg tacatccatc gatgattacg aaggtgacga atcagatggt
      181 gacagaatat acgacgaaat cgacgaaaaa gaatacaggg ccagaaagcg ccaggaattg
      241 ctgcatgacg atgctgtagt tgacgccgat ggtgtaggct acgtcgatcg tggtgtcgaa
      301 gaagattgga gggaagttga taacagttca agtgatgaag acactggaaa ccttgctagc
      361 aaggattcta aaaggaagaa gaatataaaa agggagaaag accaccagat tacagatatg
      421 ctgcgaactc aacattccaa atcaacactg ctagcacatg caaaaaaatc ccaaaaaaaa
      481 agtatcccca tcgataattt tgatgacatt cttggtgagt ttgaatctgg tgaagtagaa
      541 aaacccaata ttttactgcc atccaaatta agggaaaatt taaattcttc gccgactagt
      601 gaattttgag gaattcaaag gcctacgtcg acgagctcaa ctagtgcggc cgctttcgaa
      661 tctagagcct gcagtctcga gcggccgcat cgtgactgac tgacgatctg cctcgcgcgt
      721 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
      781 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
      841 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtataa ttcttgaaga
      901 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
      961 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     1021 taaatacatt caaatatgta tccgctcatg anacaataac cntgataaat gcttcaataa
     1081 tattgaaaaa ggaaangtat gagtattca
//
