LOCUS       sequence-31190-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-NOG1
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             49..66
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             79..99
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(799..817)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      813..860
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ctgaatattt tgtttacttt aagaaggaga tataccatgg gcagcagcca tcatcatcat
       61 catcacagca gcggcagaga aaacttgtat ttccagggct taccttctat taatcctcat
      121 aaaaaaacaa ttatattatc aggagcacca aatgtaggta aatcatcttt tatgaatatt
      181 gtatcaagag ccaatgtaga tgttcaatcc tattctttta ctacaaaaaa tttatatgta
      241 ggacattttg atcataaatt aaacaaatat caaattatag atactccagg tttattagat
      301 agagcattcg aaaacagaaa cactattgag atgactacta ttacagcact tgctcatatt
      361 aacggagtta tactctttat tatagatata agcgaacaat gtggattaac cataaaagag
      421 caaattaatt tattctattc tattaaatca gtcttttcca ataaatctat tgttatcgga
      481 tttaataaaa tcgacaaatg caatatggac agcttatcta ttgataataa attattaata
      541 aaacaaattc ttgataatgt gaaaaatcct attaaattct cttcatttag tacacttaca
      601 ggagttggag tagaacaagc caaaataact gcatgtgagt tgctaaaaaa tgatcaagct
      661 gaatctatcc tattggatca agaacaattg ctaaacacca aattgggtga aacaaaaaac
      721 tgatgacgaa gcttgcggcc gcactcgagg atccggctgc taacaaagcc cgaaaggaag
      781 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac
      841 gggtcttgag gggttttttg ctgaaggagg aactatatcc gga
//
