LOCUS       sequence-311901-        5717 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  tNCS-ePTS1 (2micron).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Grewal PS, Samson JA, Baker JJ, Choi B, Dueber JE
  TITLE     Peroxisome compartmentalization of a toxic enzyme improves alkaloid 
            production.
  JOURNAL   Nat Chem Biol. 2020 Oct 12. pii: 10.1038/s41589-020-00668-4. doi: 
            10.1038/s41589-020-00668-4.
  PUBMED    33046851
REFERENCE   2  (bases 1 to 5717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2020 Oct 12. pii: 10.1038/s41589-020-00668-4. doi: 
            10.1038/s41589-020-00668-4."
COMMENT     Sequence Label: pPSG830
FEATURES             Location/Qualifiers
     source          1..5717
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..667
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     537..560
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     promoter        1687..1874
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             1875..2537
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     rep_origin      2752..3734
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(3792..4607)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3792..4607)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4515..4534
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     4599..4618
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      4832..5420
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5321..5340
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcattatcaa tactgccatt tcaaagaata cgtaaataat taatagtagt gattttccta
       61 actttattta gtcaaaaaat tagcctttta attctgctgt aacccgtaca tgcccaaaat
      121 agggggcggg ttacacagaa tatataacat cgtaggtgtc tgggtgaaca gtttattcct
      181 ggcatccact aaatataatg gagcccgctt tttaagctgg catccagaaa aaaaaagaat
      241 cccagcacca aaatattgtt ttcttcacca accatcagtt cataggtcca ttctcttagc
      301 gcaactacag agaacagggg cacaaacagg caaaaaacgg gcacaacctc aatggagtga
      361 tgcaacctgc ctggagtaaa tgatgacaca aggcaattga cccacgcatg tatctatctc
      421 attttcttac accttctatt accttctgct ctctctgatt tggaaaaagc tgaaaaaaaa
      481 ggttgaaacc agttccctga aattattccc ctacttgact aataagtata taaagacggt
      541 aggtattgat tgtaattctg taaatctatt tcttaaactt cttaaattct acttttatag
      601 ttagtctttt ttttagtttt aaaacaccaa gaacttagtt tcgaataaac acacataaac
      661 aaacaaaaga tctatggaag aaactgtaat gttatatcac gaattggaag tcgcagcctc
      721 agctgacgaa gtatggtctg tcgaaggttc tcctgaatta ggtttacatt taccagattt
      781 gttaccagct ggaatattcg caaagtttga gataactgga gatggtggag aaggttctat
      841 tttggacatg acatttccac ctggtcaatt ccctcatcat tatagagaga agttcgtttt
      901 cttcgaccat aagaatagat ataagttggt tgaacaaatt gacggtgatt tctttgattt
      961 gggagtaacc tactacatgg acactattag ggtcgtcgca acaggtcctg attcttgtgt
     1021 catcaaatca acaactgaat atcatgttaa accagaattt gctaagattg ttaaaccttt
     1081 aatcgataca gttccattag ccattatgtc agaggcaata gctaaggtag tattggaaaa
     1141 taagcacaaa tcttctgagg gatccggttc attgggaaga ggtagaagat ccaaattgta
     1201 ataactcgag tggcagtgct tttaactaag aattattagt cttttctgct tattttttca
     1261 tcatagttta gaacacttta tattaacgaa tagtttatga atctatttag gtttaaaaat
     1321 tgatacagtt ttataagtta ctttttcaaa gactcgtgct gtctattgca taatgcactg
     1381 gaaggggaaa aaaaaggtgc acacgcgtgg ctttttcttg aatttgcagt ttgaaaaatg
     1441 ctggaaatct gctcgtcagt ggtgctcaca ctgacgaatc atgtacagat cataccgatg
     1501 actgcctggc gactcacaac taagcaagac agccggaacc agcgccggcg aacaccactg
     1561 catatatggc atatcacaac agtccacgtc tcaagcagtt acagagatgt tacgaaccac
     1621 tagtgcactg cagtacaaac acagtccttt cccgcaattt tctttttcta ttactcttgg
     1681 cctcctctag tacactctat atttttttat gcctcggtaa tgattttcat tttttttttt
     1741 ccacctagcg gatgactctt tttttttctt agcgattggc attatcacat aatgaattat
     1801 acattatata aagtaatgtg atttcttcga agaatatact aaaaaatgag caggcaagat
     1861 aaacgaaggc aaagatgaca gagcagaaag ccctagtaaa gcgtattaca aatgaaacca
     1921 agattcagat tgcgatctct ttaaagggtg gtcccctagc gatagagcac tcgatcttcc
     1981 cagaaaaaga ggcagaagca gtagcagaac aggccacaca atcgcaagtg attaacgtcc
     2041 acacaggtat agggtttctg gaccatatga tacatgctct ggccaagcat tccggctggt
     2101 cgctaatcgt tgagtgcatt ggtgacttac acatagacga ccatcacacc actgaagact
     2161 gcgggattgc tctcggtcaa gcttttaaag aggccctagg ggccgtgcgt ggagtaaaaa
     2221 ggtttggatc aggatttgcg cctttggatg aggcactttc cagagcggtg gttgatcttt
     2281 cgaacaggcc gtacgcagtt gtcgaacttg gtttgcaaag ggagaaagta ggtgatctct
     2341 cttgcgagat gatcccgcat tttcttgaaa gctttgcaga ggctagcaga attaccctcc
     2401 acgttgattg tctgcgaggc aagaatgatc atcaccgtag tgagagtgcg ttcaaggctc
     2461 ttgcggttgc cataagagaa gccacctcgc ccaatggtac caacgatgtt ccctccacca
     2521 aaggtgttct tatgtagtga caccgattat ttaaagctgc tgcatacgat atatatacat
     2581 gtgtatatat gtatacctat gaatgtcagt aagtatgtat acgaacagta tgatactgaa
     2641 gatgacaagg taatgcatca ttctatacgt gtcattctga acgaggcgcg ctttcctttt
     2701 ttctttttgc tttttctttt tttttctctt gaactcgacg gatcatagag taacgaagca
     2761 tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag
     2821 aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt ttaccaacga
     2881 agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta atttttcaaa
     2941 caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg ctattttacc
     3001 aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag cgctattttt
     3061 ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat gcagtctctt
     3121 gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg gtgtctattt
     3181 tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact agcgaagctg
     3241 cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga tgtggattgc
     3301 gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca gaaaattatg
     3361 aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac attttcgtat
     3421 tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa agagtaatac
     3481 tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag gagcgaaagg
     3541 tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag atacttttga
     3601 gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt ccggtgcgtt
     3661 tttggttttt tgaaagtgcg tcatcagagc gcttttggtt ttcaaaagcg ctctgaagtt
     3721 cctatacttt ctagctagag aataggaact tcccgagcgg ccgcgtgtta caaccaatta
     3781 accaattctg attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca
     3841 ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg
     3901 aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca
     3961 tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca
     4021 tgagtgacga ctgaatccgg tgagaatggc aaaagcttat gcatttcttt ccagacttgt
     4081 tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc
     4141 attcgtgatt gcgcctgagc gaggcgaaat acgcgatcgc tgttaaaagg acaattacaa
     4201 acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct
     4261 gaatcaggat attcttctaa tacctggaat gctgttttcc cggggatcgc agtggtgagt
     4321 aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc
     4381 gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca
     4441 tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct
     4501 gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa
     4561 tttaatcgcg gcctggagca agacgtttcc cgttgaatat ggctcataac accccttgta
     4621 ttactgttta tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca
     4681 atgtaacatc agagattttg agacacaacg tggctttgtt gaataaatcg aacttttgct
     4741 gagttgaagg atcagtcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     4801 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     4861 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     4921 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     4981 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     5041 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     5101 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     5161 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     5221 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     5281 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     5341 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     5401 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     5461 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgtg
     5521 cggccgcccc tgaattcgca tctagactga tgagacgtgg tagagccaca aacagccggt
     5581 acaagcaacg atctccagga ccatctgaat catgcgcgga tgacacgaac tcacgacggc
     5641 gatcacagac attaacccac agtacagaca ctgcgacaac gtggcaattc gtcgcaatac
     5701 aacgcagttc gagttta
//
