LOCUS       sequence-311904-       11160 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of nontargeting shRNA, GFP expression, based 
            on Addgene 12247.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11160)
  TITLE     Human Citron shRNA and controls
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11160)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Lentiviral nontargeting shRNA GFP
FEATURES             Location/Qualifiers
     source          1..11160
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1299..1532
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1717..1761
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2146..3324
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2377..3315
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3272..3292
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3372..3489
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      3574..3583
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3580..3582,3583..3585,3586..4296)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3580..3582,3583..3585,3586..4296)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3625..3646)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3886..3905)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4233..4254
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4371..4959
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4424..4444)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4842..4853)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    join(5127..5145,5146..5168,5169..5187,5188..5210,5211..5229,
                     5230..5252,5253..5271,5272..5294,5295..5313,5314..5336,
                     5337..5355,5356..5378,5379..5397)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        5426..5640
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     5561..5580
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    complement(5722..5755)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             5775..5955
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(5980..5998)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5981..5998)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6288..6307)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6521..6540)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6640..6662
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6700..6718)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6786..6890
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6891..6959,6960..7751)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7109..7128)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7922..8510
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8411..8430
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8664..8681
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8751..8771)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8756..9085
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8936..9071
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10219..10284
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10414..10434
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10859..10993
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10896..10915)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10950..10969
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatct cgacggtatc gatcacgaga ctagcctcga
     2041 ccatcgcgat gtcgacgata agctttgcaa agatggataa agttttaaac agagaggaat
     2101 ctttgcagct aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt
     2161 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     2221 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     2281 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     2341 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
     2401 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg
     2461 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct
     2521 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc
     2581 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta
     2641 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta
     2701 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
     2761 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
     2821 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
     2881 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
     2941 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
     3001 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
     3061 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt
     3121 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     3181 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     3241 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     3301 tttcttccat ttcaggtgtc gtgaggaatt tcgacattta aatttaatta atctcgacgg
     3361 tatcggttaa cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     3421 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     3481 caaaatttta tcgatcacga gactagcctc gaggtttaaa ctacgggatc ttcgaaggcc
     3541 taagcttacg cgcgcgtcct agcgctaccg gtcgccacca tggtgagcaa gggcgaggag
     3601 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     3661 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     3721 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     3781 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     3841 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     3901 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     3961 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     4021 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     4081 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     4141 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     4201 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     4261 gccgggatca ctctcggcat ggacgagctg tacaagtccg gactcagatc tcgactagct
     4321 agtagctagc tagctagtcg agctcaagct tcggggacta gtcatatgat aatcaacctc
     4381 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     4441 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     4501 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     4561 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     4621 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     4681 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     4741 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
     4801 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     4861 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     4921 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcggtacgta tggccaggta
     4981 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     5041 gggggactgg aagggctaat tcactcccaa cgaagacaag atgggatcaa ttcaccatgc
     5101 tagtggatcc ccctcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     5161 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     5221 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     5281 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
     5341 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     5401 gtgaaagtcg gggctgcagg aattcgaacg ctgacgtcat caacccgctc caaggaatcg
     5461 cgggcccagt gtcactaggc gggaacaccc agcgcgcgtg cgccctggca ggaagatggc
     5521 tgtgagggac aggggagtgg cgccctgcaa tatttgcatg tcgctatgtg ttctgggaaa
     5581 tcaccataaa cgtgaaatgt ctttggattt gggaatctta taagttctgt atgagaccac
     5641 gcgccctctc gcttgggcga gagtaagttc aagagactta ctctcgccca agcgagattt
     5701 ttacgatacc gtcgcatggg aataacttcg tatagcatac attatacgaa gttatgctgc
     5761 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     5821 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     5881 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     5941 ggaaaatctc tagcagcatc tagaattaat tccgtgtatt ctatagtgtc acctaaatcg
     6001 tatgtgtatg atacataagg ttatgtatta attgtagccg cgttctaacg acaatatgta
     6061 caagcctaat tgtgtagcat ctggcttact gaagcagacc ctatcatctc tctcgtaaac
     6121 tgccgtcaga gtcggtttgg ttggacgaac cttctgagtt tctggtaacg ccgtcccgca
     6181 cccggaaatg gtcagcgaac caatcagcag ggtcatcgct agccagatcc tctacgccgg
     6241 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga
     6301 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt
     6361 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc
     6421 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca
     6481 ggagtcgcat aagggagagc gtcgaatggt gcactctcag tacaatctgc tctgatgccg
     6541 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     6601 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     6661 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     6721 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     6781 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     6841 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     6901 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     6961 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     7021 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     7081 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     7141 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     7201 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     7261 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     7321 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     7381 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     7441 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     7501 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     7561 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     7621 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     7681 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     7741 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     7801 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     7861 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     7921 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     7981 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     8041 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     8101 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     8161 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     8221 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     8281 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     8341 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     8401 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     8461 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     8521 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     8581 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     8641 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     8701 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg
     8761 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     8821 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     8881 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     8941 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     9001 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     9061 ttttggaggc ctaggctttt gcaaaaagct tggacacaag acaggcttgc gagatatgtt
     9121 tgagaatacc actttatccc gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat
     9181 gtgaaatact ggtttttagt gcgccagatc tctataatct cgcgcaacct attttcccct
     9241 cgaacacttt ttaagccgta gataaacagg ctgggacact tcacatgagc gaaaaataca
     9301 tcgtcacctg ggacatgttg cagatccatg cacgtaaact cgcaagccga ctgatgcctt
     9361 ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact
     9421 ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt atttccagct acgatcacga
     9481 caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt
     9541 tattgatgac ctggtggata ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa
     9601 agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt
     9661 tgttgatatc ccgcaagata cctggattga acagccgtgg gatatgggcg tcgtattcgt
     9721 cccgccaatc tccggtcgct aatcttttca acgcctggca ctgccgggcg ttgttctttt
     9781 taacttcagg cgggttacaa tagtttccag taagtattct ggaggctgca tccatgacac
     9841 aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa acctcgacgc
     9901 tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa
     9961 ccatccctca ctggtatcgc atgattaacc gtctgatgtg gatctggcgc ggcattgacc
    10021 cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg
    10081 atgatttata cgatacggtg attggctacc gtggcggcaa ctggatttat gagtgggccc
    10141 cggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
    10201 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
    10261 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
    10321 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
    10381 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
    10441 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
    10501 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
    10561 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
    10621 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
    10681 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
    10741 gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
    10801 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
    10861 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
    10921 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
    10981 tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc
    11041 ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc
    11101 tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt
//
