LOCUS       sequence-311908-       11463 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus for doxycycline inducible expression of nontargeting 
            shRNA, mKate marker, based on Addgene 11652.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11463)
  TITLE     Human Citron shRNA and controls
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Dox human Citron shRNA 1 mKate
FEATURES             Location/Qualifiers
     source          1..11463
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1299..1532
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1717..1761
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1945..2062
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2131..2342
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2362..2371
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2368..3066
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     misc_feature    3072..3639
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3090..3641
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3234..3251)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3461..3480
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4265..4285
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4388..4582
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     primer_bind     complement(4721..4737)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4722..4738)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    4758..5346
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4811..4831)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5229..5240)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    5514..5532
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5556..5574
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5598..5616
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5640..5658
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5682..5700
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        5729..5943
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     5864..5883
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    complement(6025..6058)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             6078..6258
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6283..6301)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6284..6301)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6591..6610)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6824..6843)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6943..6965
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7003..7021)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7089..7193
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7194..7262,7263..8054)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7412..7431)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8225..8813
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8714..8733
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8967..8984
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9054..9074)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9059..9388
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9239..9374
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10522..10587
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10717..10737
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11162..11296
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11199..11218)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11253..11272
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccagacc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     1981 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2041 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2101 ccggctcgag tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
     2161 agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa
     2221 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
     2281 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
     2341 aggtgtcgtg acgcgggatc cgccaccatg gtgagcgagc tgattaagga gaacatgcac
     2401 atgaagctgt acatggaggg caccgtgaac aaccaccact tcaagtgcac atccgagggc
     2461 gaaggcaagc cctacgaggg cacccagacc atgagaatca aggcggtcga gggcggccct
     2521 ctccccttcg ccttcgacat cctggctacc agcttcatgt acggcagcaa aaccttcatc
     2581 aaccacaccc agggcatccc cgacttcttt aagcagtcct tccccgaggg cttcacatgg
     2641 gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga caccagcctc
     2701 caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttccc atccaacggc
     2761 cctgtgatgc agaagaaaac actcggctgg gaggcctcca ccgagaccct gtaccccgct
     2821 gacggcggcc tggaaggcag agccgacatg gccctgaagc tcgtgggcgg gggccacctg
     2881 atctgcaact tgaagaccac atacagatcc aagaaacccg ctaagaacct caagatgccc
     2941 ggcgtctact atgtggacag aagactggaa agaatcaagg aggccgacaa agagacctac
     3001 gtcgagcagc acgaggtggc tgtggccaga tactgcgacc tccctagcaa actggggcac
     3061 agatgaggaa ttccgccccc ccccccctaa cgttactggc cgaagccgct tggaataagg
     3121 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     3181 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     3241 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
     3301 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
     3361 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
     3421 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     3481 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     3541 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aacgtctagg ccccccgaac
     3601 cacggggacg tggttttcct ttgaaaaaca cgatgataat accatggcta gactggacaa
     3661 gagcaaagtc ataaacggcg ctctggaatt actcaatgga gtcggtatcg aaggcctgac
     3721 gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct accctgtact ggcacgtgaa
     3781 gaacaagcgg gccctgctcg atgccctgcc aatcgagatg ctggacaggc atcataccca
     3841 cttctgcccc ctggaaggcg agtcatggca agactttctg cggaacaacg ccaagtcatt
     3901 ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg catctcggca cccgcccaac
     3961 agagaaacag tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc
     4021 cctggagaac gcactgtacg ctctgtccgc cgtgggccac tttacactgg gctgcgtatt
     4081 ggaggaacag gagcatcaag tagcaaaaga ggaaagagag acacctacca ccgattctat
     4141 gcccccactt ctgagacaag caattgagct gttcgaccgg cagggagccg aacctgcctt
     4201 ccttttcggc ctggaactaa tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg
     4261 gtcgccaaaa aagaagagaa aggtcgacgg cggtggtgct ttgtctcctc agcactctgc
     4321 tgtcactcaa ggaagtatca tcaagaacaa ggagggcatg gatgctaagt cactaactgc
     4381 ctggtcccgg acactggtga ccttcaagga tgtatttgtg gacttcacca gggaggagtg
     4441 gaagctgctg gacactgctc agcagatcgt gtacagaaat gtgatgctgg agaactataa
     4501 gaacctggtt tccttgggtt atcagcttac taagccagat gtgatcctcc ggttggagaa
     4561 gggagaagag ccctggctgg tggagagaga aattcaccaa gagacccatc ctgattcaga
     4621 gactgcattt gaaatcaaat catcagttta aggatctcga ctagctagta gctagctagc
     4681 tagtcgagct caagcttcga attcgatatc aagcttatcg cgataccgtc gacctcgacc
     4741 tcgactagtc atatgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     4801 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     4861 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     4921 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     4981 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     5041 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     5101 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     5161 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     5221 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     5281 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     5341 ccccgcatcg gtacgtatgg ccaggtacct ttaagaccaa tgacttacaa ggcagctgta
     5401 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     5461 agacaagatg ggatcaattc accatgctag tggatccccc tcgagtttac cactccctat
     5521 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     5581 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     5641 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     5701 aaagtgaaag tcggggctgc aggaattcga acgctgacgt catcaacccg ctccaaggaa
     5761 tcgcgggccc agtgtcacta ggcgggaaca cccagcgcgc gtgcgccctg gcaggaagat
     5821 ggctgtgagg gacaggggag tggcgccctg caatatttgc atgtcgctat gtgttctggg
     5881 aaatcaccat aaacgtgaaa tgtctttgga tttgggaatc ttataagttc tgtatgagac
     5941 cacgcgccca tggaaggcac tatttctcaa ttcaagagat tgagaaatag tgccttccat
     6001 tttttacgat accgtcgcat gggaataact tcgtatagca tacattatac gaagttatgc
     6061 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     6121 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     6181 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     6241 tgtggaaaat ctctagcagc atctagaatt aattccgtgt attctatagt gtcacctaaa
     6301 tcgtatgtgt atgatacata aggttatgta ttaattgtag ccgcgttcta acgacaatat
     6361 gtacaagcct aattgtgtag catctggctt actgaagcag accctatcat ctctctcgta
     6421 aactgccgtc agagtcggtt tggttggacg aaccttctga gtttctggta acgccgtccc
     6481 gcacccggaa atggtcagcg aaccaatcag cagggtcatc gctagccaga tcctctacgc
     6541 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc
     6601 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg
     6661 cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc
     6721 accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat
     6781 gcaggagtcg cataagggag agcgtcgaat ggtgcactct cagtacaatc tgctctgatg
     6841 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     6901 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     6961 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     7021 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     7081 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     7141 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     7201 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     7261 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     7321 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     7381 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     7441 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     7501 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     7561 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     7621 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     7681 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     7741 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     7801 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     7861 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     7921 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     7981 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     8041 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     8101 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     8161 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     8221 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     8281 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     8341 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     8401 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8461 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8521 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     8581 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     8641 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     8701 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     8761 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     8821 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     8881 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     8941 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     9001 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt
     9061 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     9121 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     9181 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     9241 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     9301 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     9361 cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat
     9421 gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact
     9481 catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc
     9541 cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat
     9601 acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc
     9661 cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac cgggtgcgtt
     9721 actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca
     9781 cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat
     9841 cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc
     9901 aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta
     9961 tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt
    10021 cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct
    10081 ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga
    10141 cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga
    10201 cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta
    10261 aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg
    10321 acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg
    10381 acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt tatgagtggg
    10441 ccccggatct ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct
    10501 acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta
    10561 ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag
    10621 tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat
    10681 gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa
    10741 gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat
    10801 agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag
    10861 aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac
    10921 atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa
    10981 aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat
    11041 agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt
    11101 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
    11161 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
    11221 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    11281 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
    11341 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc
    11401 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt
    11461 agt
//
