LOCUS       sequence-31191-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-UBC13 (2R0J)
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             64..84
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(607..625)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      621..668
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(843..848,849..865,866..871))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgcatcatc atcatcatca cagcagcggc
       61 agagaaaact tgtatttcca gggcattccg cgtcgcatca ccaaagaaac gcagaatctg
      121 gcgaacgagc cacctcccgg cattatggcc gtgccggttc cagaaaatta tcgtcatttt
      181 aacatattaa tcaatggtcc tgatgggact ccgtacgagg gaggcacata taagttggaa
      241 cttttcctcc ccgaacaata cccgatggag ccacctaaag tccggtttct gaccaaaatt
      301 tatcacccga acatcgacaa gctgggtcgc atatgcttag atattttgaa agacaaatgg
      361 tctcccgcac ttcagatcag aacggtactg ctcagtattc aagctctgtt atcatccccg
      421 gaaccagatg accctctgga ttcgaaggtg gcggagcatt tcaaacagga taaaaatgac
      481 gccgaacacg ttgcacgaca gtggaacaag atttacgcta ataacaattg atgacgaagc
      541 ttgcggccgc actcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg
      601 ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg
      661 gttttttgct gaaaggagga actatatccg gatatcccgc aagaggcccg gcagtaccgg
      721 cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc
      781 attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc
      841 gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cga
//
