LOCUS       sequence-311914-       10818 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A multicistronic vector with both CAGGS promoter-driven AsCpf1 and 
            U6 promoter-driven single guide RNA (sgRNA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10818)
  AUTHORS   Ma H, Jeppesen JF, Jaenisch R
  TITLE     Human T Cells Expressing a CD19 CAR-T Receptor Provide Insights into
            Mechanisms of Human CD19-Positive beta Cell Destruction.
  JOURNAL   Cell Rep Med. 2020 Sep 22;1(6):100097. doi: 
            10.1016/j.xcrm.2020.100097. eCollection 2020 Sep 22.
  PUBMED    33205073
REFERENCE   2  (bases 1 to 10818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Med."; date: "2020-09-22"; volume: "1(6):100097. doi"; pages: " 
            10.1016/j.xcrm.2020.100097. eCollection 2020 Sep 22"
COMMENT     Sequence Label: CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector
FEATURES             Location/Qualifiers
     source          1..10818
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        306..583
                     /label=chicken beta-actin promoter
     intron          584..1600
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1524..1546
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1608..1627
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1667..1687
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1712..5629
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     regulatory      5349..5358
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5630..5677
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5684..5764
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             5774..5827
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5828..5830,5831..6541)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(5828..5830,5831..6541)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5870..5891)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6131..6150)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6478..6499
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6569..6586)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6575..6782
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   6791..6931
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   6791..6920
                     /label=AAV2 ITR
     rep_origin      7006..7461
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7093..7112)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7303..7324
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7478..7497)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7597..7619
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7657..7675)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7743..7847
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7848..7916,7917..8708)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8066..8085)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8879..9467
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9368..9387
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        9529..9777
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     9529..9549
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     9700..9719
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        9821..9896
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     10120..10139
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10260..10721
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
      361 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
      421 gggggggcgc gcgccrggsg gggsggggsg gggsgrgggg sggggcgggg cgaggcggag
      481 aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg
      541 gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg
      601 ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac
      661 cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg
      721 cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg aggggctccg
      781 ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg
      841 gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg
      901 gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg
      961 cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc
     1021 agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc cccgagttgc
     1081 tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt
     1141 gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc ctcgggccgg
     1201 ggagggctcg ggggaggggc gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc
     1261 gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc
     1321 ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg
     1381 ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc
     1441 gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg acggctgcct
     1501 tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc
     1561 ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt
     1621 tattgtgctg tctcatcatt ttggcaaaga ccggtccacc atggccccaa agaagaagcg
     1681 gaaggtcggt atccacggag tcccagcagc cacacagttc gagggcttta ccaacctgta
     1741 tcaggtgagc aagacactgc ggtttgagct gatcccacag ggcaagaccc tgaagcacat
     1801 ccaggagcag ggcttcatcg aggaggacaa ggcccgcaat gatcactaca aggagctgaa
     1861 gcccatcatc gatcggatct acaagaccta tgccgaccag tgcctgcagc tggtgcagct
     1921 ggattgggag aacctgagcg ccgccatcga ctcctataga aaggagaaaa ccgaggagac
     1981 aaggaacgcc ctgatcgagg agcaggccac atatcgcaat gccatccacg actacttcat
     2041 cggccggaca gacaacctga ccgatgccat caataagaga cacgccgaga tctacaaggg
     2101 cctgttcaag gccgagctgt ttaatggcaa ggtgctgaag cagctgggca ccgtgaccac
     2161 aaccgagcac gagaacgccc tgctgcggag cttcgacaag tttacaacct acttctccgg
     2221 cttttatgag aacaggaaga acgtgttcag cgccgaggat atcagcacag ccatcccaca
     2281 ccgcatcgtg caggacaact tccccaagtt taaggagaat tgtcacatct tcacacgcct
     2341 gatcaccgcc gtgcccagcc tgcgggagca ctttgagaac gtgaagaagg ccatcggcat
     2401 cttcgtgagc acctccatcg aggaggtgtt ttccttccct ttttataacc agctgctgac
     2461 acagacccag atcgacctgt ataaccagct gctgggagga atctctcggg aggcaggcac
     2521 cgagaagatc aagggcctga acgaggtgct gaatctggcc atccagaaga atgatgagac
     2581 agcccacatc atcgcctccc tgccacacag attcatcccc ctgtttaagc agatcctgtc
     2641 cgataggaac accctgtctt tcatcctgga ggagtttaag agcgacgagg aagtgatcca
     2701 gtccttctgc aagtacaaga cactgctgag aaacgagaac gtgctggaga cagccgaggc
     2761 cctgtttaac gagctgaaca gcatcgacct gacacacatc ttcatcagcc acaagaagct
     2821 ggagacaatc agcagcgccc tgtgcgacca ctgggataca ctgaggaatg ccctgtatga
     2881 gcggagaatc tccgagctga caggcaagat caccaagtct gccaaggaga aggtgcagcg
     2941 cagcctgaag cacgaggata tcaacctgca ggagatcatc tctgccgcag gcaaggagct
     3001 gagcgaggcc ttcaagcaga aaaccagcga gatcctgtcc cacgcacacg ccgccctgga
     3061 tcagccactg cctacaaccc tgaagaagca ggaggagaag gagatcctga agtctcagct
     3121 ggacagcctg ctgggcctgt accacctgct ggactggttt gccgtggatg agtccaacga
     3181 ggtggacccc gagttctctg cccggctgac cggcatcaag ctggagatgg agccttctct
     3241 gagcttctac aacaaggcca gaaattatgc caccaagaag ccctactccg tggagaagtt
     3301 caagctgaac tttcagatgc ctacactggc ctctggctgg gacgtgaata aggagaagaa
     3361 caatggcgcc atcctgtttg tgaagaacgg cctgtactat ctgggcatca tgccaaagca
     3421 gaagggcagg tataaggccc tgagcttcga gcccacagag aaaaccagcg agggctttga
     3481 taagatgtac tatgactact tccctgatgc cgccaagatg atcccaaagt gcagcaccca
     3541 gctgaaggcc gtgacagccc actttcagac ccacacaacc cccatcctgc tgtccaacaa
     3601 tttcatcgag cctctggaga tcacaaagga gatctacgac ctgaacaatc ctgagaagga
     3661 gccaaagaag tttcagacag cctacgccaa gaaaaccggc gaccagaagg gctacagaga
     3721 ggccctgtgc aagtggatcg acttcacaag ggattttctg tccaagtata ccaagacaac
     3781 ctctatcgat ctgtctagcc tgcggccatc ctctcagtat aaggacctgg gcgagtacta
     3841 tgccgagctg aatcccctgc tgtaccacat cagcttccag agaatcgccg agaaggagat
     3901 catggatgcc gtggagacag gcaagctgta cctgttccag atctataaca aggactttgc
     3961 caagggccac cacggcaagc ctaatctgca cacactgtat tggaccggcc tgttttctcc
     4021 agagaacctg gccaagacaa gcatcaagct gaatggccag gccgagctgt tctaccgccc
     4081 taagtccagg atgaagagga tggcacaccg gctgggagag aagatgctga acaagaagct
     4141 gaaggatcag aaaaccccaa tccccgacac cctgtaccag gagctgtacg actatgtgaa
     4201 tcacagactg tcccacgacc tgtctgatga ggccagggcc ctgctgccca acgtgatcac
     4261 caaggaggtg tctcacgaga tcatcaagga taggcgcttt accagcgaca agttcttttt
     4321 ccacgtgcct atcacactga actatcaggc cgccaattcc ccatctaagt tcaaccagag
     4381 ggtgaatgcc tacctgaagg agcaccccga gacacctatc atcggcatcg atcggggcga
     4441 gagaaacctg atctatatca cagtgatcga ctccaccggc aagatcctgg agcagcggag
     4501 cctgaacacc atccagcagt ttgattacca gaagaagctg gacaacaggg agaaggagag
     4561 ggtggcagca aggcaggcct ggtctgtggt gggcacaatc aaggatctga agcagggcta
     4621 tctgagccag gtcatccacg agatcgtgga cctgatgatc cactaccagg ccgtggtggt
     4681 gctggagaac ctgaatttcg gctttaagag caagaggacc ggcatcgccg agaaggccgt
     4741 gtaccagcag ttcgagaaga tgctgatcga taagctgaat tgcctggtgc tgaaggacta
     4801 tccagcagag aaagtgggag gcgtgctgaa cccataccag ctgacagacc agttcacctc
     4861 ctttgccaag atgggcaccc agtctggctt cctgttttac gtgcctgccc catatacatc
     4921 taagatcgat cccctgaccg gcttcgtgga ccccttcgtg tggaaaacca tcaagaatca
     4981 cgagagccgc aagcacttcc tggagggctt cgactttctg cactacgacg tgaaaaccgg
     5041 cgacttcatc ctgcacttta agatgaacag aaatctgtcc ttccagaggg gcctgcccgg
     5101 ctttatgcct gcatgggata tcgtgttcga gaagaacgag acacagtttg acgccaaggg
     5161 cacccctttc atcgccggca agagaatcgt gccagtgatc gagaatcaca gattcaccgg
     5221 cagataccgg gacctgtatc ctgccaacga gctgatcgcc ctgctggagg agaagggcat
     5281 cgtgttcagg gatggctcca acatcctgcc aaagctgctg gagaatgacg attctcacgc
     5341 catcgacacc atggtggccc tgatccgcag cgtgctgcag atgcggaact ccaatgccgc
     5401 cacaggcgag gactatatca acagccccgt gcgcgatctg aatggcgtgt gcttcgactc
     5461 ccggtttcag aacccagagt ggcccatgga cgccgatgcc aatggcgcct accacatcgc
     5521 cctgaagggc cagctgctgc tgaatcacct gaaggagagc aaggatctga agctgcagaa
     5581 cggcatctcc aatcaggact ggctggccta catccaggag ctgcgcaaca aaaggccggc
     5641 ggccacgaaa aaggccggcc aggcaaaaaa gaaaaaggga tcctacccat acgatgttcc
     5701 agattacgct tatccctacg acgtgcctga ttatgcatac ccatatgatg tccccgacta
     5761 tgccggcagt ggagagggca gaggaagtct gctaacatgc ggtgacgtcg aggagaatcc
     5821 tggcccagtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     5881 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     5941 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     6001 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     6061 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     6121 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     6181 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     6241 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     6301 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     6361 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     6421 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     6481 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     6541 ggaattctaa ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     6601 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     6661 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     6721 ggtggggtgg ggcaggacag caagggggag gattgggaag agaatagcag gcatgctggg
     6781 gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg
     6841 ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca
     6901 gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg
     6961 catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg
     7021 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     7081 ccttagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     7141 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     7201 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga
     7261 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     7321 ctggaacaac actcaactct atctcgggct attcttttga tttataaggg attttgccga
     7381 tttcggtcta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     7441 aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat
     7501 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     7561 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     7621 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     7681 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     7741 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     7801 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     7861 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     7921 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     7981 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     8041 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     8101 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     8161 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     8221 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     8281 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     8341 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     8401 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     8461 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     8521 ctggctggtt tattgctgat aaatctggag ccggtgagcg tggaagccgc ggtatcattg
     8581 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     8641 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     8701 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     8761 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     8821 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     8881 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     8941 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     9001 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     9061 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     9121 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     9181 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     9241 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     9301 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     9361 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     9421 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     9481 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgtga gggcctattt
     9541 cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat aattggaatt
     9601 aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt
     9661 cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat gcttaccgta
     9721 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccgta
     9781 atttctactc ttgtagatgt cttcgagaag actttttttt gttttagagc tagaaatagc
     9841 aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt
     9901 ttgttttaga gctagaaata gcaagttaaa ataaggctag tccgttttta gcgcgtgcgc
     9961 caattctgca gacaaatggc tctaggcatg ccgacggcga ggatctcgtc gtgacccatg
    10021 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
    10081 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
    10141 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
    10201 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga ggggatccgc
    10261 tgtaagtctg cagaaattga tgatctatta aacaataaag atgtccacta aaatggaagt
    10321 ttttcctgtc atactttgtt aagaagggtg agaacagagt acctacattt tgaatggaag
    10381 gattggagct acgggggtgg gggtggggtg ggattagata aatgcctgct ctttactgaa
    10441 ggctctttac tattgcttta tgataatgtt tcatagttgg atatcataat ttaaacaagc
    10501 aaaaccaaat taagggccag ctcattcctc ccactcatga tctatagatc tatagatctc
    10561 tcgtgggatc attgtttttc tcttgattcc cactttgtgg ttctaagtac tgtggtttcc
    10621 aaatgtgtca gtttcatagc ctgaagaacg agatcagcag cctctgttcc acatacactt
    10681 cattctcagt attgttttgc caagttctaa ttccatcaga agctgggagc tccaccgcgg
    10741 tggcggccgc tctagaacta gttattaata gtaatcaatt acggggtcat tagttcatag
    10801 cccatatatg gagttccg
//
