LOCUS       sequence-31198-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-CyP5
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(810..828)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      824..871
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tgaatatttt gtttacttta agaaggagat ataccatgca tcatcatcat catcacagca
       61 gcggcagaga aaacttgtat ttccagggct ctacccgtgg caaagtcgta ctgcacacat
      121 ctctgggtga tctggacgtg gaactgtggg cgcgcgagtg cccactggcc tgtcgtaatt
      181 ttgttcagct gtgcctggaa gggtactatg tcaacactat tttccatcgc gtagtgaaag
      241 attttatcgt tcagggaggc gaccctacag gtaccgggcg tggaggcgca gatacgactt
      301 tcgacggtaa accgtttgat gtcgaaacac acccacgcct gaaattccgt taccgcggcc
      361 tggtgggtgt agctaatctg gggcgttcat ccaaagatgc ggagaacgac gaacgcggac
      421 gtagcctggg gaccaatggt aaccagtttt tcattacgct ggcccgcgca gatgttctga
      481 ataacgctta taccctgttt ggcaaagtga ctggtcatac actgtacaat ctgatgaaat
      541 tcaacgacct ggaggtcggg aaagaagatc gtcctatgac gccgccgttt atcaaaagcg
      601 ttgacgtact gtggaatcca tttgaagatc tggtgcctcg ccgtctgccg gatgcgccgc
      661 cagcccaaaa agacgagcgc aaacgtgcac gcgctgcggg aggccgtacc actgaaggtc
      721 gcgcccgttc gtgatgacga agcttgcggc cgcactcgag gatccggctg ctaacaaagc
      781 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
      841 gncctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc
      901 cgca
//
