LOCUS       sequence-311982-        8070 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 S (surface 
            glycoprotein).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8070)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 8070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4133341
FEATURES             Location/Qualifiers
     source          1..8070
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        306..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             4402..4422
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             4423..4464
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     polyA_signal    4517..4638
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4528..4547)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4582..4601
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      4753..4799
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      4829..4965
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    5010..5081
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(5307..5895)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5387..5406)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(6641..6709,6710..7501)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6859..6878)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      7514..7650
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    7802..7873
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(7878..7924)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    7942..7990
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catgctgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatca gatctttgtc
      541 gatcctacca tccactcgac acacccgcca gcggccgcca ccatgttcgt ctttcttgtg
      601 ttactccccc tggtttcgtc ccagtgcgtg aacttgacta cgcgaaccca actgccgcca
      661 gcatatacaa atagtttcac ccgtggagta tactaccctg ataaagtgtt tagatcaagc
      721 gtactacact ctactcagga cctgtttctc cccttcttct ccaatgtcac atggtttcat
      781 gcgatccacg ttagcggcac caacgggaca aagcgctttg acaaccctgt gctgccattc
      841 aatgatggtg tgtattttgc ttctaccgag aagagcaaca taattcgggg ctggatcttc
      901 ggaactaccc tcgacagtaa aactcagtca ttgctgattg tgaataacgc cacaaatgtc
      961 gttatcaagg tctgtgaatt tcaattctgc aacgatccat tccttggggt gtattaccat
     1021 aaaaataata agtcctggat ggagagcgaa tttagggtat actcgtctgc caacaattgt
     1081 actttcgagt atgtgtctca gcccttctta atggatctgg aaggtaaaca gggcaacttt
     1141 aagaacctcc gcgagtttgt attcaaaaat atagacggat actttaagat ttattccaag
     1201 cacacaccaa tcaatttggt gcgggatctc ccgcaagggt tcagcgctct ggaacctcta
     1261 gtggacctcc ccatcggcat taacatcaca agattccaga cgctgttagc cctgcataga
     1321 tcatacctta ctcccggtga cagtagttct gggtggaccg caggcgcggc tgcatattac
     1381 gtgggatatt tgcagcctcg gacgtttctg ctgaaataca acgagaacgg caccattaca
     1441 gatgccgtgg attgcgccct tgacccccta agcgaaacta agtgtacgct taaaagcttc
     1501 accgtcgaga agggaattta ccagacttcc aactttaggg tccagccgac agaatcaata
     1561 gttaggttcc caaatatcac caatttgtgc ccttttgggg aagtcttcaa tgctacacga
     1621 tttgcttcgg tttatgcctg gaacaggaag cgtatctcaa attgcgtcgc ggactacagc
     1681 gtgctctata atagtgcaag tttctcgacc tttaaatgtt atggcgtgtc cccgaccaaa
     1741 ctaaatgacc tctgttttac aaacgtttac gccgattctt ttgtgattcg cggtgatgag
     1801 gtacgccaaa tcgcaccggg ccagactggc aagattgccg attacaacta caagttgcct
     1861 gacgacttca ccggatgtgt tatagcctgg aatagcaata atctggattc taaggtcgga
     1921 ggtaactata actacctgta tagactgttc cgtaagtcaa acctgaaacc ctttgagcgg
     1981 gacatctcca cagagattta ccaagcaggg tctaccccct gcaatggggt tgaaggattc
     2041 aattgctact ttccattaca gagctacggg ttccagccta cgaacggggt tggttaccag
     2101 ccttataggg tagtggtgct gtcgttcgaa ctgttgcacg ctccagctac agtgtgcggg
     2161 cctaaaaaat caactaacct ggttaaaaat aagtgtgtta attttaactt caatggctta
     2221 accggaactg gagtgctcac ggagtcaaac aagaaatttc taccgtttca gcaattcggg
     2281 agggatatcg cagacactac ggacgcggtc agagatccac agacccttga gatccttgac
     2341 ataacaccct gctctttcgg aggtgtctcc gtaattacac caggcactaa tacctctaat
     2401 caggtggccg tgctttatca ggatgttaac tgtacagagg tcccggtggc tatccatgcc
     2461 gaccaattga cacctacgtg gcgcgtctat tctaccggct ccaacgtctt ccagacccgg
     2521 gctggatgct tgataggcgc agaacacgta aacaactctt acgaatgtga tattcccatt
     2581 ggagccggga tctgcgcaag ctaccagacc caaacaaaca gtcccagacg ggctcgtagc
     2641 gtggcctctc agagcataat agcctatacc atgtcccttg gcgcggagaa tagtgtggca
     2701 tactcaaata atagcatcgc tatccccact aactttacta tttccgtgac gactgagatt
     2761 ctgccagtgt ctatgactaa gacatccgtg gattgcacaa tgtatatttg tggggattcc
     2821 acagagtgta gcaacttgtt gctgcaatat ggatcattct gtacgcagct gaatcgcgcc
     2881 ctcaccggca tcgctgtgga acaggacaag aacactcaag aggtctttgc tcaggtgaag
     2941 caaatctaca agaccccacc tataaaagac ttcgggggtt ttaacttctc gcagattctc
     3001 cccgacccca gcaagccttc aaaaaggagc tttattgagg acctgttgtt caacaaggtt
     3061 actttagccg atgccggttt tatcaaacag tacggcgact gtcttggcga tatagcagcc
     3121 cgggatctga tttgcgcaca aaaattcaat ggccttaccg tactgccacc ccttctcacc
     3181 gacgaaatga tcgcgcagta cacctccgca ctgctggctg ggaccatcac ttcgggatgg
     3241 acctttgggg ctggtgctgc tttgcaaatt cccttcgcca tgcagatggc ctatcgattc
     3301 aatggcatag gggtaacaca gaacgtattg tatgaaaatc aaaagctaat cgctaaccag
     3361 tttaattcag ccattggtaa gatccaggat tcactctcca gtacagcctc tgccctggga
     3421 aagctccagg acgtggtcaa ccagaacgcg caggcattga atacactcgt caaacagctg
     3481 agtagcaatt tcggcgcaat cagtagtgtc ttaaacgata tactgagtag gcttgataaa
     3541 gtagaggccg aggtccagat tgacagacta atcacggggc gtcttcagag tttacagacc
     3601 tatgtgaccc aacagctgat ccgagcagcg gaaattcgag caagcgcgaa cctggccgct
     3661 actaaaatga gtgagtgcgt gctgggccaa agtaaacgag tcgacttttg cggcaaaggg
     3721 tatcatctca tgtctttccc tcagtccgcc cctcacggtg ttgttttcct ccacgtgaca
     3781 tacgttcccg cgcaggaaaa gaattttact acggcacccg ctatttgcca tgacggaaag
     3841 gctcattttc cacgggaggg agtgttcgtt tcaaacggga cacactggtt tgtaactcag
     3901 cggaactttt atgagcctca aatcatcacg acagacaata ccttcgtgag tggcaactgc
     3961 gatgtagtca taggcattgt gaataacact gtttatgatc cacttcagcc ggaactcgac
     4021 agcttcaaag aggaattgga taagtacttt aaaaatcata ccagtccaga cgtagatctc
     4081 ggcgatatat cgggcattaa cgccagtgtg gtcaatatac aaaaggagat tgacagactg
     4141 aatgaagtag ctaagaacct caacgaatct ctgattgacc tgcaagagct agggaagtac
     4201 gagcagtata tcaaatggcc atggtacata tggctgggtt ttatcgcggg tcttattgcc
     4261 atagtcatgg tgacgattat gttgtgctgt atgacctcct gctgttcttg tttgaaaggc
     4321 tgctgctcct gtggtagctg ttgcaaattc gatgaagatg actctgaacc cgtgttgaaa
     4381 ggcgttaagc tacactacac agaaaacctt tactttcagt cggggaaacc tatacccaat
     4441 ccacttctcg gcttagattc tacctaagct agcttgactg actgagatac agcgtacctt
     4501 cagctcacag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     4561 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     4621 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     4681 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat tggcccatct
     4741 ctatcggtat cgtagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgt
     4801 gcccctcggg ccggattgct atctaccggc attggcgcag aaaaaaatgc ctgatgcgac
     4861 gctgcgcgtc ttatactccc acatatgcca gattcagcaa cggatacggc ttccccaact
     4921 tgcccacttc catacgtgtc ctccttacca gaaatttatc cttaaggtcg tcagctatcc
     4981 tgcaggcgat ctctcgattt cgatcaagac attcctttaa tggtcttttc tggacaccac
     5041 taggggtcag aagtagttca tcaaactttc ttccctccct aatctcattg gttaccttgg
     5101 gctatcgaaa cttaattaac cagtcaagtc agctacttgg cgagatcgac ttgtctgggt
     5161 ttcgactacg ctcagaattg cgtcagtcaa gttcgatctg gtccttgcta ttgcacccgt
     5221 tctccgatta cgagtttcat ttaaatcatg tgagcaaaag gccagcaaaa ggccaggaac
     5281 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     5341 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     5401 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     5461 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     5521 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     5581 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     5641 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     5701 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     5761 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     5821 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     5881 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     5941 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     6001 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     6061 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     6121 tccatagttg catttaaatt tccgaactct ccaaggccct cgtcggaaaa tcttcaaacc
     6181 tttcgtccga tccatcttgc aggctacctc tcgaacgaac tatcgcaagt ctcttggccg
     6241 gccttgcgcc ttggctattg cttggcagcg cctatcgcca ggtattactc caatcccgaa
     6301 tatccgagat cgggatcacc cgagagaagt tcaacctaca tcctcaatcc cgatctatcc
     6361 gagatccgag gaatatcgaa atcggggcgc gcctggtgta ccgagaacga tcctctcagt
     6421 gcgagtctcg acgatccata tcgttgcttg gcagtcagcc agtcggaatc cagcttggga
     6481 cccaggaagt ccaatcgtca gatattgtac tcaagcctgg tcacggcagc gtaccgatct
     6541 gtttaaacct agatattgat agtctgatcg gtcaacgtat aatcgagtcc tagcttttgc
     6601 aaacatctat caagagacag gatcagcagg aggctttcgc atgagtattc aacatttccg
     6661 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     6721 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgcg cgagtgggtt acatcgaact
     6781 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgct ttccaatgat
     6841 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     6901 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtatt caccagtcac
     6961 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     7021 gagtgataac actgcggcca acttacttct gacaacgatt ggaggaccga aggagctaac
     7081 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     7141 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     7201 cttgcgtaaa ctattaactg gcgaactact tactctagct tcccggcaac agttgataga
     7261 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     7321 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     7381 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     7441 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     7501 accgattcta ggtgcattgg cgcagaaaaa aatgcctgat gcgacgctgc gcgtcttata
     7561 ctcccacata tgccagattc agcaacggat acggcttccc caacttgccc acttccatac
     7621 gtgtcctcct taccagaaat ttatccttaa gatcccgaat cgtttaaact cgactctggc
     7681 tctatcgaat ctccgtcgtt tcgagcttac gcgaacagcc gtggcgctca tttgctcgtc
     7741 gggcatcgaa tctcgtcagc tatcgtcagc ttaccttttt ggcagcgatc gcggctcccg
     7801 acatcttgga ccattagctc cacaggtatc ttcttccctc tagtggtcat aacagcagct
     7861 tcagctacct ctcaattcaa aaaacccctc aagacccgtt tagaggcccc aaggggttat
     7921 gctatcaatc gttgcgttac acacacaaaa aaccaacaca catccatctt cgatggatag
     7981 cgattttatt atctaactgc tgatcgagtg tagccagatc tagtaatcaa ttacggggtc
     8041 attagttcat agcccatata tggagttccg
//
