LOCUS       sequence-311983-        4563 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 nsp9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4563)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4134405
FEATURES             Location/Qualifiers
     source          1..4563
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        306..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             919..939
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             940..957
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    1010..1131
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1021..1040)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1075..1094
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      1246..1292
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      1322..1458
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    1503..1574
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(1800..2388)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1880..1899)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(3134..3202,3203..3994)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3352..3371)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      4007..4143
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    4295..4366
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(4371..4417)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    4435..4483
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catgctgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatca gatctttgtc
      541 gatcctacca tccactcgac acacccgcca gcggccgcca ccatgaatga gcttagtcct
      601 gttgcactac gacagatgtc ttgtgctgcc ggtactacac aaactgcttg cactgatgac
      661 aatgcgttag cttactacaa cacaacaaag ggaggtaggt ttgtacttgc actgttatcc
      721 gatttacagg atttgaaatg ggctagattc cctaagagtg atggaactgg tactatctat
      781 acagaactgg aaccaccttg taggtttgtt acagacacac ctaaaggtcc taaagtgaag
      841 tatttatact ttattaaagg attaaacaac ctaaatagag gtatggtact tggtagttta
      901 gctgccacag tacgtctaga aaacctttac tttcagtcgc accatcacca tcaccattaa
      961 gctagcttga ctgactgaga tacagcgtac cttcagctca cagacatgat aagatacatt
     1021 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     1081 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     1141 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
     1201 taaaacctct acaaatgtgg tattggccca tctctatcgg tatcgtagca taaccccttg
     1261 gggcctctaa acgggtcttg aggggttttt tgtgcccctc gggccggatt gctatctacc
     1321 ggcattggcg cagaaaaaaa tgcctgatgc gacgctgcgc gtcttatact cccacatatg
     1381 ccagattcag caacggatac ggcttcccca acttgcccac ttccatacgt gtcctcctta
     1441 ccagaaattt atccttaagg tcgtcagcta tcctgcaggc gatctctcga tttcgatcaa
     1501 gacattcctt taatggtctt ttctggacac cactaggggt cagaagtagt tcatcaaact
     1561 ttcttccctc cctaatctca ttggttacct tgggctatcg aaacttaatt aaccagtcaa
     1621 gtcagctact tggcgagatc gacttgtctg ggtttcgact acgctcagaa ttgcgtcagt
     1681 caagttcgat ctggtccttg ctattgcacc cgttctccga ttacgagttt catttaaatc
     1741 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     1801 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     1861 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     1921 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     1981 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     2041 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     2101 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     2161 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     2221 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     2281 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     2341 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     2401 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     2461 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     2521 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     2581 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcatttaa atttccgaac
     2641 tctccaaggc cctcgtcgga aaatcttcaa acctttcgtc cgatccatct tgcaggctac
     2701 ctctcgaacg aactatcgca agtctcttgg ccggccttgc gccttggcta ttgcttggca
     2761 gcgcctatcg ccaggtatta ctccaatccc gaatatccga gatcgggatc acccgagaga
     2821 agttcaacct acatcctcaa tcccgatcta tccgagatcc gaggaatatc gaaatcgggg
     2881 cgcgcctggt gtaccgagaa cgatcctctc agtgcgagtc tcgacgatcc atatcgttgc
     2941 ttggcagtca gccagtcgga atccagcttg ggacccagga agtccaatcg tcagatattg
     3001 tactcaagcc tggtcacggc agcgtaccga tctgtttaaa cctagatatt gatagtctga
     3061 tcggtcaacg tataatcgag tcctagcttt tgcaaacatc tatcaagaga caggatcagc
     3121 aggaggcttt cgcatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     3181 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     3241 tcagttgggt gcgcgagtgg gttacatcga actggatctc aacagcggta agatccttga
     3301 gagttttcgc cccgaagaac gctttccaat gatgagcact tttaaagttc tgctatgtgg
     3361 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     3421 tcagaatgac ttggttgagt attcaccagt cacagaaaag catcttacgg atggcatgac
     3481 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     3541 tctgacaacg attggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     3601 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     3661 tgacaccacg atgcctgtag caatggcaac aaccttgcgt aaactattaa ctggcgaact
     3721 acttactcta gcttcccggc aacagttgat agactggatg gaggcggata aagttgcagg
     3781 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     3841 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     3901 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     3961 tgagataggt gcctcactga ttaagcattg gtaaccgatt ctaggtgcat tggcgcagaa
     4021 aaaaatgcct gatgcgacgc tgcgcgtctt atactcccac atatgccaga ttcagcaacg
     4081 gatacggctt ccccaacttg cccacttcca tacgtgtcct ccttaccaga aatttatcct
     4141 taagatcccg aatcgtttaa actcgactct ggctctatcg aatctccgtc gtttcgagct
     4201 tacgcgaaca gccgtggcgc tcatttgctc gtcgggcatc gaatctcgtc agctatcgtc
     4261 agcttacctt tttggcagcg atcgcggctc ccgacatctt ggaccattag ctccacaggt
     4321 atcttcttcc ctctagtggt cataacagca gcttcagcta cctctcaatt caaaaaaccc
     4381 ctcaagaccc gtttagaggc cccaaggggt tatgctatca atcgttgcgt tacacacaca
     4441 aaaaaccaac acacatccat cttcgatgga tagcgatttt attatctaac tgctgatcga
     4501 gtgtagccag atctagtaat caattacggg gtcattagtt catagcccat atatggagtt
     4561 ccg
//
