LOCUS       sequence-311987-        5493 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 N (nucleocapsid).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5493)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 5493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4134505
FEATURES             Location/Qualifiers
     source          1..5493
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        306..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1840..1860
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1861..1887
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    1940..2061
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1951..1970)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2005..2024
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      2176..2222
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      2252..2388
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    2433..2504
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(2730..3318)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2810..2829)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(4064..4132,4133..4924)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4282..4301)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      4937..5073
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    5225..5296
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(5301..5347)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    5365..5413
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catgctgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatca gatctttgtc
      541 gatcctacca tccactcgac acacccgcca gcggccgcca ccatgtctga taatggacca
      601 cagaaccaac gcaatgcacc ccgaatcact tttggcggtc cgtccgacag taccgggagc
      661 aaccagaacg gcgaaagatc gggagctcgt tcaaaacagc ggaggcctca agggttaccc
      721 aataacacgg ccagctggtt cacagcgttg acccagcatg gcaaggagga cctcaagttc
      781 cctcggggtc agggggtccc aataaataca aacagttccc ctgatgacca aattggatac
      841 tatagacgcg ctactaggag gatccgtggc ggggatggaa aaatgaagga cctttctccc
      901 agatggtact tttattacct gggtactggc ccagaggccg gtctaccgta tggagcaaac
      961 aaagatggca ttatttgggt ggccacggaa ggagcactga ataccccaaa ggatcacatc
     1021 ggcacccgga atccagccaa caatgctgct atcgttctgc aactccccca ggggacaaca
     1081 ttgcctaaag gcttctatgc cgagggcagt agaggtggca gccaggcgtc ctcacgcagc
     1141 tcttctcgat ctcggaacag ctcaagaaat tccactcccg ggagttcccg aggaaccagc
     1201 ccagcaagga tggctggcaa tggtggagat gccgctctgg ccctacttct gctggaccgc
     1261 ctcaatcagt tagaatcaaa gatgtcgggg aaggggcagc aacagcaggg acagaccgta
     1321 actaaaaaga gtgcagcgga ggcctctaaa aagcctcggc agaaacgtac ggcaacaaag
     1381 gcttacaacg tgacccaggc ctttggccgt agaggacccg aacagacaca agggaacttt
     1441 ggtgaccaag agttgataag gcagggaacc gactacaaac actggcctca gatcgcccaa
     1501 ttcgcaccga gcgcgagcgc atttttcgga atgtcacgaa ttggcatgga ggtgacacct
     1561 tcgggaacct ggctgacata cactggcgcc ataaagctcg atgacaaaga tcccaacttc
     1621 aaggatcaag tcatcttgct gaataagcat attgacgcgt ataaaacttt tccacccacg
     1681 gaaccgaaga aggacaagaa aaagaaagct gatgagacac aggcgcttcc acaaaggcag
     1741 aaaaagcagc agacggttac tctactgccc gccgcagacc tagatgactt ctccaaacag
     1801 cttcagcagt ctatgagtag cgccgactca acccaagctg aaaaccttta ctttcagtcg
     1861 tacccctatg acgtaccaga ttatgcgtaa gctagcttga ctgactgaga tacagcgtac
     1921 cttcagctca cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1981 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     2041 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     2101 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tattggccca
     2161 tctctatcgg tatcgtagca taaccccttg gggcctctaa acgggtcttg aggggttttt
     2221 tgtgcccctc gggccggatt gctatctacc ggcattggcg cagaaaaaaa tgcctgatgc
     2281 gacgctgcgc gtcttatact cccacatatg ccagattcag caacggatac ggcttcccca
     2341 acttgcccac ttccatacgt gtcctcctta ccagaaattt atccttaagg tcgtcagcta
     2401 tcctgcaggc gatctctcga tttcgatcaa gacattcctt taatggtctt ttctggacac
     2461 cactaggggt cagaagtagt tcatcaaact ttcttccctc cctaatctca ttggttacct
     2521 tgggctatcg aaacttaatt aaccagtcaa gtcagctact tggcgagatc gacttgtctg
     2581 ggtttcgact acgctcagaa ttgcgtcagt caagttcgat ctggtccttg ctattgcacc
     2641 cgttctccga ttacgagttt catttaaatc atgtgagcaa aaggccagca aaaggccagg
     2701 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     2761 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     2821 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     2881 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     2941 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     3001 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     3061 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     3121 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     3181 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     3241 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     3301 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     3361 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     3421 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     3481 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     3541 tcatccatag ttgcatttaa atttccgaac tctccaaggc cctcgtcgga aaatcttcaa
     3601 acctttcgtc cgatccatct tgcaggctac ctctcgaacg aactatcgca agtctcttgg
     3661 ccggccttgc gccttggcta ttgcttggca gcgcctatcg ccaggtatta ctccaatccc
     3721 gaatatccga gatcgggatc acccgagaga agttcaacct acatcctcaa tcccgatcta
     3781 tccgagatcc gaggaatatc gaaatcgggg cgcgcctggt gtaccgagaa cgatcctctc
     3841 agtgcgagtc tcgacgatcc atatcgttgc ttggcagtca gccagtcgga atccagcttg
     3901 ggacccagga agtccaatcg tcagatattg tactcaagcc tggtcacggc agcgtaccga
     3961 tctgtttaaa cctagatatt gatagtctga tcggtcaacg tataatcgag tcctagcttt
     4021 tgcaaacatc tatcaagaga caggatcagc aggaggcttt cgcatgagta ttcaacattt
     4081 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     4141 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcgcgagtgg gttacatcga
     4201 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gctttccaat
     4261 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     4321 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt attcaccagt
     4381 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     4441 catgagtgat aacactgcgg ccaacttact tctgacaacg attggaggac cgaaggagct
     4501 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     4561 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     4621 aaccttgcgt aaactattaa ctggcgaact acttactcta gcttcccggc aacagttgat
     4681 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     4741 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     4801 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     4861 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     4921 gtaaccgatt ctaggtgcat tggcgcagaa aaaaatgcct gatgcgacgc tgcgcgtctt
     4981 atactcccac atatgccaga ttcagcaacg gatacggctt ccccaacttg cccacttcca
     5041 tacgtgtcct ccttaccaga aatttatcct taagatcccg aatcgtttaa actcgactct
     5101 ggctctatcg aatctccgtc gtttcgagct tacgcgaaca gccgtggcgc tcatttgctc
     5161 gtcgggcatc gaatctcgtc agctatcgtc agcttacctt tttggcagcg atcgcggctc
     5221 ccgacatctt ggaccattag ctccacaggt atcttcttcc ctctagtggt cataacagca
     5281 gcttcagcta cctctcaatt caaaaaaccc ctcaagaccc gtttagaggc cccaaggggt
     5341 tatgctatca atcgttgcgt tacacacaca aaaaaccaac acacatccat cttcgatgga
     5401 tagcgatttt attatctaac tgctgatcga gtgtagccag atctagtaat caattacggg
     5461 gtcattagtt catagcccat atatggagtt ccg
//
