LOCUS       sequence-311998-        4680 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLCO2B1_STOP
FEATURES             Location/Qualifiers
     source          1..4680
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2805..2904)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2921..2940)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2922..2940)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2945..2961)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2945..2961)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3063..3082)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3074..3883
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3141..3160)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4030..4618
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4519..4538
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgggcc caaggatcgg accagcagga gaggtgcctc aggtgccaga
      721 caaggagacc aaggccacaa tgggaaccga gaacacacca ggaggcaagg caagcccaga
      781 ccctcaggat gtgcgccctt ccgtgttcca caatatcaag ctgtttgtgc tgtgccacag
      841 cctgctgcag ctggcccagc tgatgatctc tggctacctg aagagctcca tcagcaccgt
      901 ggagaagagg ttcggcctgt ctagccagac atctggcctg ctggccagct ttaacgaagt
      961 gggcaatacc gccctgatcg tgttcgtgtc ttactttggc agccgcgtgc accggccaag
     1021 aatgatcggc tatggagcaa tcctggtggc cctggcagga ctgctgatga ccctgcctca
     1081 cttcatctct gagccatacc ggtatgataa cacaagccct gaggacatgc cacaggactt
     1141 caaggccagc ctgtgcctgc caaccacatc cgccccagca tctgccccta gcaacggaaa
     1201 ttgttcctct tataccgaga cacagcacct gtccgtggtg ggcatcatgt tcgtggcaca
     1261 gaccctgctg ggagtgggag gcgtgcccat ccagcctttc ggcatcagct acatcgacga
     1321 ttttgcccac aactctaata gcccactgta tctgggcatc ctgttcgcag tgaccatgat
     1381 gggaccagga ctggcctttg gcctgggatc tctgatgctg cggctgtacg tggacatcaa
     1441 tcagatgcct gagggcggca tcagcctgac aatcaaggat ccaagatggg tgggagcatg
     1501 gtggctgggc ttcctgatcg cagcaggagc agtggccctg gcagccatcc catatttctt
     1561 tttcccaaag gagatgccca aggagaagcg ggagctgcag tttcggagaa aggtgctggc
     1621 agtgaccgac tccccagcaa gaaagggcaa ggattcccca tctaagcagt cccccggcga
     1681 gtctaccaag aagcaggacg gcctggtgca gatcgcccct aacctgacag tgatccagtt
     1741 catcaaggtg tttccaaggg tgctgctgca gaccctgagg cacccaatct tcctgctggt
     1801 ggtgctgagc caggtgtgcc tgagctccat ggcagcagga atggccatct tcctgcccaa
     1861 gtttctggag agacagttca gcatcacagc ctcctacgcc aatctgctga tcggctgtct
     1921 gtcctttcca tctgtgatcg tgggaatcgt ggtgggaggc gtgctggtga agaggctgca
     1981 cctgggacca gtgggatgcg gcgccctgtg cctgctgggc atgctgctgt gcctgttttt
     2041 ctccctgcct ctgtttttca tcggctgttc tagccaccag atcgccggca tcacccacca
     2101 gacatctgcc cacccaggac tggagctgag cccttcctgc atggaggcct gcagctgtcc
     2161 cctggacggc ttcaaccccg tgtgcgatcc atccaccaga gtggagtaca tcacaccttg
     2221 ccacgccggc tgttcctctt gggtggtgca ggacgccctg gataactccc aggtgtttta
     2281 taccaattgc tcttgcgtgg tggagggcaa tcctgtgctg gccggcagct gcgactccac
     2341 atgttctcac ctggtggtgc cattcctgct gctggtgagc ctgggatccg ccctggcatg
     2401 cctgacccac acaccttcct ttatgctgat cctgaggggc gtgaagaagg aggataagac
     2461 cctggccgtg ggcatccagt tcatgtttct gcgcatcctg gcctggatgc catcccccgt
     2521 gatccacggc tctgccatcg acaccacatg cgtgcactgg gcactgagct gcggcaggcg
     2581 cgccgtgtgc cgctactata acaatgatct gctgaggaac cgcttcatcg gcctgcagtt
     2641 tttctttaag accggctccg tgatctgttt tgcactggtg ctggccgtgc tgaggcagca
     2701 ggacaaggag gcaaggacaa aggagagcag aagctcccca gcagtggagc agcagctgct
     2761 ggtgtccgga ccaggcaaga agcctgagga ttctagagtg taagacccag ctttcttgta
     2821 caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat
     2881 cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta
     2941 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat
     3001 tgcacaagat aaaataatat catcatgaac aataaaactg tctgcttaca taaacagtaa
     3061 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa
     3121 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
     3181 gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
     3241 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
     3301 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
     3361 cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
     3421 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
     3481 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
     3541 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
     3601 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
     3661 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg
     3721 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
     3781 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
     3841 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg
     3901 gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa
     3961 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     4021 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     4081 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     4141 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     4201 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     4261 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     4321 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     4381 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     4441 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4501 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4561 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4621 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
