LOCUS       sequence-31201-e         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-mACP
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             76..96
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(493..511)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      507..554
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(729..734,735..751,752..757))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(784..802)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ttcctctgaa atattttgtt tactttaaga aggagatata ccatgcatca tcatcatcat
       61 cacagcagcg gcagagaaaa cttgtatttc cagggcgcaa atgaaaccac taagcaagag
      121 tctccagtag tggatacaga cattaacgct gttacgaatt atatcgtcgg tatgtgccag
      181 aaatttctgc aaaaaggcga aaaggtgacc cctagttcaa aattagaaga gctgcgcact
      241 cgggaagatc gtctgtggga ctgtttagat acagtagagt tcgttctgga tgtggaagaa
      301 atttttgacg tcaccgttcc ggatgaggta gcggacaact tccagacgct gcaggaaatc
      361 gccgattttg tggtcagcga gcgtgcaaaa gcgggtaagt ttatgaaaga ccaatgatga
      421 cgaagcttgc ggccgcactc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt
      481 tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
      541 tgaggggttt tttgctgaaa ggaggaacta tatccggata tcccgcaaga ggcccggcag
      601 taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat
      661 gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa
      721 actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa
      781 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
      841 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
      901 tacattcaaa tatgtatccg ctcatgagac aataacccct gataaatgct tcaataatat
      961 tg
//
