LOCUS       sequence-312016-        3831 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC16A13_STOP
FEATURES             Location/Qualifiers
     source          1..3831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1956..2055)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2072..2091)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2073..2091)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2096..2112)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2096..2112)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2214..2233)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2225..3034
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2292..2311)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3181..3769
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3670..3689
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatggccc ggagaaccga gccacctgat ggaggatggg gatgggtggt
      721 ggtgctgtcc gccttctttc agtctgccct ggtgttcggc gtgctgcggt cctttggcgt
      781 gttctttgtg gagttcgtgg cagcctttga ggagcaggca gcaagagtgt cctggatcgc
      841 ctctatcgga atcgcagtgc agcagttcgg aagcccagtg ggaagcgccc tgtccaccaa
      901 gtttggacca aggcctgtgg tcatgacagg aggaatcctg gccgccctgg gaatgctgct
      961 ggccagcttc gccacatccc tgacccacct gtacctgtcc atcggactgc tgtctggaag
     1021 cggatgggca ctgacattcg cccccaccct ggcctgcctg tcttgttact tcagcaggcg
     1081 ccggtccctg gcaaccggac tggccctgac aggcgtgggc ctgagctcct tcacctttgc
     1141 ccctttcttt cagtggctgc tgtctcacta cgcatggagg ggaagcctgc tgctggtgtc
     1201 tgccctgagc ctgcacctgg tggcctgcgg cgccctgctg cgcccaccct ctctggcaga
     1261 ggacccagca gtgggaggac cacgggccca gctgacaagc ctgctgcacc acggcccttt
     1321 cctgagatat acagtggccc tgaccctgat caacacaggc tactttatcc catatctgca
     1381 cctggtggcc cacctgcagg acctggattg ggacccactg ccagcagcct tcctgctgag
     1441 cgtggtggcc atctccgatc tggtgggcag ggtggtgtcc ggatggctgg gcgacgcagt
     1501 gcctggacca gtgacccgcc tgctgatgct gtggaccaca ctgacaggcg tgtctctggc
     1561 cctgtttcct gtggcacagg caccaaccgc cctggtggcc ctggcagtgg catacggctt
     1621 cacatccggc gccctggcac cactggcctt ttctgtgctg cctgagctga tcggcaccag
     1681 aaggatctat tgcggcctgg gcctgctgca gatgatcgag agcatcggag gactgctggg
     1741 acctccactg tccggatacc tgcgggatgt gaccggcaat tatacagcca gcttcgtggt
     1801 ggcaggagcc tttctgctgt ccggatctgg catcctgctg accctgccac acttcttttg
     1861 tttctctacc acaaccagcg gaccacagga tctggtgacc gaggccctgg acacaaaggt
     1921 gcccctgcct aaggagggcc tggaggagga ctaagaccca gctttcttgt acaaagttgg
     1981 cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa
     2041 taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat
     2101 agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga
     2161 taaaataata tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg
     2221 tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc
     2281 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta
     2341 tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt
     2401 tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct
     2461 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat
     2521 ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt
     2581 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt
     2641 taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt
     2701 tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga
     2761 aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact
     2821 tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg
     2881 aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc
     2941 ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt
     3001 gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca
     3061 ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa
     3121 cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3181 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3241 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3301 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     3361 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3421 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3481 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     3541 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     3601 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     3661 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     3721 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     3781 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt t
//
