LOCUS       sequence-31202-e         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-GAPDH
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             65..85
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tattttgttt actttaagaa ggagatatac catgcatcat catcatcatc acagcagcgg
       61 cagagaaaac ttgtatttcc agggcacttt gggaattaac ggtttcggac gtattggtcg
      121 tttagttttg agagcctgta tggaaagaaa tgacattaca gtagtagcaa taaatgatcc
      181 attcatggac gttgaatata tggcttacct cttgaagtat gattcagttc atggtaactt
      241 caatggtaca gttgaggtat caggcaagga tttatgcatc aacggtaagg tagtaaaggt
      301 attccaagct aaggacccag cagaaatccc atggggtgct tcaggtgctc aaattgtatg
      361 cgagtcaact ggtgtattca caacagagga gaaggcttca ttgcatctta aaggcggtgc
      421 caagaaggtc attatctctg ctccaccaaa ggacaatgtc ccaatgtatg tcatgggagt
      481 aaataacact gaatatgacc catcaaaatt caacgttatc tctaacgctt cttgcacaac
      541 aaactgtctt gctcctttgg ctaagattat taatgacaag tttggaattg ttgaaggttt
      601 gatgacaact gttcactctc ttactgctaa ccaattaact gttgatggtc caagcaaggg
      661 tggcaaggac tggagagcag gaagatgcgc tgggaacaac attattccag caagtactgg
      721 agcagctaag gccgtaggta aggtcatccc agctttgaat ggaaagctca caggtatggc
      781 catccgtgtc ccaactccag atgtttccgt tgttgattta acatgcaagc ttgcaaaacc
      841 agcctctata gaggaaatct atcaagccgt taaggaagca tccaatggtc caatgaaggg
      901 aattatgggc tacacaagcg atgatgtcgt ctcgaccgat ttcat
//
