LOCUS       sequence-31203-e         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-TRX3
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..90
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(514..532)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      528..575
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(750..755,756..772,773..778))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(805..823)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctgaatattt tgtttacttt aagaaggaga tataccatgc atcatcatca tcatcacagc
       61 agcggcagag aaaacttgta tttccagggc cagagcatct acatcgagtt aaagaacacc
      121 ggctctctga accaagtgtt ttcctcgacg cagaacagct ccattgttat caaattcggt
      181 gcggtctggt gtaaaccgtg caataagatt aaagagtact tcaaaaacca gctgaactac
      241 tactacgtga ccctggtcga tatcgacgta gacattcacc caaagttgaa tgaccagcat
      301 aatatcaaag ccctgcctac ttttgagttt tacttcaacc tgaacaacga gtgggtgctt
      361 gttcacacag tagaaggggc aaatcaaaat gacattgaga aagctttcca gaagtactgc
      421 ctggaaaaag cgaaatgatg acgaagcttg cggccgcact cgaggatccg gctgctaaca
      481 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc
      541 ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat
      601 atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt
      661 gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg
      721 ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac
      781 atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
      841 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
      901 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
      961 gataaatgc
//
