LOCUS       sequence-312066-        3870 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC17A2_STOP
FEATURES             Location/Qualifiers
     source          1..3870
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1995..2094)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2111..2130)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2112..2130)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2135..2151)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2135..2151)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2253..2272)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2264..3073
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2331..2350)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3220..3808
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3709..3728
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatggacg gcaagccagc aaccaggaag ggacctgatt tttgctccct
      721 gcgctacggc ctggccctga tcatgcactt ctctaacttt accatgatca cacagcgggt
      781 gagcctgtcc atcgccatca tcgccatggt gaacaccaca cagcagcagg gcctgtccaa
      841 tgcatctacc gagggaccag tggcagacgc cttcaacaat agctccatca gcatcaagga
      901 gtttgatacc aaggccagcg tgtaccagtg gagcccagag acacagggca tcatcttttc
      961 tagcatcaat tacggcatca tcctgacact gatcccctcc ggctatctgg ccggcatctt
     1021 cggagccaag aagatgctgg gagcaggact gctgatctcc tctctgctga ccctgttcac
     1081 acccctggcc gccgactttg gcgtgatcct ggtcatcatg gtgcggaccg tgcagggaat
     1141 ggcacaggga atggcatgga ccggccagtt cacaatctgg gcaaagtggg caccacctct
     1201 ggagagaagc aagctgacca caatcgcagg atctggaagc gccttcggct cctttatcat
     1261 cctgtgcgtg ggaggactga tcagccaggc cctgtcctgg cctttcatct tttacatctt
     1321 cggctccacc ggctgcgtgt gctgtctgct gtggtttaca gtgatctatg acgatcctat
     1381 gcaccaccca tgtatctctg tgagggagaa ggagcacatc ctgagctccc tggcacagca
     1441 gccatctagc ccaggaaggg cagtgcctat caaggcaatg gtgacatgcc tgccactgtg
     1501 ggccatcttc ctgggcttct ttagccactt ttggctgtgc accatcatcc tgacatacct
     1561 gcctacctat atctctacac tgctgcacgt gaacatcagg gacagcggcg tgctgtcctc
     1621 tctgccattc atcgcagcag catcttgtac catcctggga ggccagctgg ccgattttct
     1681 gctgagccgg aatctgctga gactgatcac agtgcgcaag ctgttcagct ccctgggcct
     1741 gctgctgcca agcatctgcg cagtggccct gccctttgtg gcatctagct acgtgatcac
     1801 catcatcctg ctgatcctga tccccggcac atccaacctg tgcgactctg gcttcatcat
     1861 caataccctg gatatcgccc ctcggtatgc cagcttcctg atgggcatct ccagaggctt
     1921 tggcctgatc gccggcatca tctcctctac cgccacaggc ttcctgatct cccaagtggg
     1981 ccccgtgtac taagacccag ctttcttgta caaagttggc attataagaa agcattgctt
     2041 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc
     2101 agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg
     2161 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaataatat catcatgaac
     2221 aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg
     2281 ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg
     2341 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga
     2401 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga
     2461 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat
     2521 ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca
     2581 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct
     2641 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg
     2701 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga
     2761 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt
     2821 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga
     2881 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga
     2941 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt
     3001 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga
     3061 tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac
     3121 ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca
     3181 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     3241 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     3301 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     3361 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     3421 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     3481 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     3541 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     3601 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     3661 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     3721 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     3781 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     3841 ggccttttgc tggccttttg ctcacatgtt
//
