LOCUS       sequence-31208-e         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-UBC13 (3E95)
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(618..636)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      632..679
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ctgaatattt tgtttacttt agaaggagat ataccatgca tcatcatcat catcacagca
       61 gcggcagaga aaacttgtat ttccagggca ttccgcgtcg catcaccaaa gaaacgcaga
      121 atctggcgaa cgagccacct cccggcatta tggccgtgcc ggttccagaa aattatcgtc
      181 attttaacat attaatcaat ggtcctgatg ggactccgta cgagggaggc acatataagt
      241 tggaactttt cctccccgaa caatacccga tggagccacc taaagtccgg tttctgacca
      301 aaatttatca cccgaacatc gacaagctgg gtcgcatatg cttagatatt ttgaaagaca
      361 aatggtctcc cgcacttcag atcagaacgg tactgctcag tattcaagct ctgttatcat
      421 ccccggaacc agatgaccct ctggattcga aggtggcgga gcatttcaaa caggataaaa
      481 atgacgccga acacgttgca cgacagtgga acaagattta cgctaataac aatgtcttgt
      541 gatgacgaag cttgcggccg cactcgagga tccggctgct aacaaagccc gaaaggaagc
      601 tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg
      661 ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatcccg caagaggccc
      721 ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg
      781 acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt
      841 gataaactac cgcattaaag cttatcgatg at
//
