LOCUS       sequence-31215-e         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SPS
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             77..97
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             340..357
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(410..428)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      424..471
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(595..614)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     805..826
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aattcctctg aatattttgt ttactttaag aaggagatat accatgcatc atcatcatca
       61 tcacagcagc ggcagagaaa acttgtattt ccagggctcc gcccggctaa gagccttgag
      121 gcagaggcag ctggaccgag ctgcggctgc tgtggaacct gatgttgttg ttaaacgaca
      181 agaagcttta gcagctgctc gactgaaaat gcaagaagaa ctaaatgcgc aagttgaaaa
      241 gcataaggaa aaactgaaac aacttgaaga agaaaaaagg agacagaaga ttgaaatgtg
      301 ggacagcatg tgatgacgaa gcttgcggcc gcactcgagc accaccacca ccaccactga
      361 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
      421 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga
      481 ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
      541 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
      601 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
      661 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
      721 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
      781 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca cactcaaccc
      841 tatctcggtc tatttc
//
