LOCUS       sequence-31218-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CAPN08
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             56..73
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             83..100
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             517..534
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(587..605)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      601..648
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(772..791)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attccctctg aatattttgt ttactttaag aaggagatat accatgggca gcagccatca
       61 tcatcatcat cacagcagcg gcctggttcc gcgtggtagt ccatacacct tatcaaaacg
      121 gattaatgga aagtggagtg gtcagagtgc tggaggatgt ggaaatttcc aagagactca
      181 caaaaataac cccatctacc aattccatat agaaaagact gggccgttac tgattgagct
      241 acgaggacca aggcaatata gcgttggatt tgaggttgta acagtttcta ctctaggaga
      301 tcctggtccc catggctttc tgaggaaatc tagtggtgac tataggtgtg ggttttgcta
      361 cctggaatta gaaaatatac cttctgggat cttcaatatc attcctagta cctttttgcc
      421 taaacaagaa ggaccttttt tcttggactt taatagtatt atccccatca agatcacaca
      481 acttcagtga tgacgaagct tgcggccgca ctcgagcacc accaccacca ccactgagat
      541 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
      601 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga
      661 actatatccg gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
      721 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
      781 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
      841 ggctcccttt agggttccga tttagtgctt tacggcaccc tcga
//
