LOCUS       sequence-31219-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: UBC64
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             60..77
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             87..104
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             635..652
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(705..723)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      719..766
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gaattcccct ctgaaatatt ttgtttactt taagaaggag atataccatg ggcagcagcc
       61 atcatcatca tcatcacagc agcggcctgg ttccgcgtgg tagtggctcg gtgcaggtca
      121 ctgaccggct gatgaaggag ctcagggata tataccgatc acagagtttc aaaggcggaa
      181 actatgcagt cgaactcgtg aatgacagtc tgtatgattg gaatgtcaaa ctcctcaaag
      241 ttgaccagga cagcgctttg cacaacgatc tccagatcct caaagagaaa gaaggagccg
      301 acttcattct acttaacttt tcctttaaag ataactttcc ctttgaccca ccatttgtca
      361 gggttgtgtc tccagtcctc tctggagggt atgttctggg cggaggggcc atctgcatgg
      421 aacttctcac caaacagggc tggagcagtg cctactccat agagtcagtg atcatgcaga
      481 tcagtgccac actggtgaag gggaaagcac gagtgcagtt tggagccaac aaatctcaat
      541 acagtctgac aagagcacag cagtcctaca agtccttggt gcagatccac gaaaaaaacg
      601 gctggtgatg acgaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc
      661 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
      721 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac
      781 tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
      841 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
      901 ttcttccc
//
