LOCUS       sequence-31221-e         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: UBC47
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(32..49)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(641..658)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(668..685)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             704..726
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    741..765
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(765..784)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(766..784)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tcctttcggg cttgttagca gccggatctc agtggtggtg gtggtggtgc tcgagttatg
       61 tagcgtatct cttggtccac tgtctggcca ttctgtcatg ttctgctctg ttggtcatat
      121 actgagtggc aatacttccc accaaggggt cggcaggatt acagtctgta agaagtgagc
      181 agatagaaag gaggacttta gaaatggtta gtgctggact ccaattatct ttcaatatgt
      241 ccaagcaaat aacaccttga ctgttaatat tacaatgata gattcttgtc cgaaatgtaa
      301 cctttggagg cttgaaggga tattctggtg taaaagtgat atcgagaaag aatacaccac
      361 cctcatacac ggatcctgga ggccctagaa tggttgatct ccattcatag atgttatcgc
      421 ctttgggacc agcactgcaa ttaggtggag ggtctaaagt gatgtccgcc agctccttct
      481 gaattctctt ggcgctggtg gagaggagtt tggagttttt gctcatgctg actttactct
      541 ccttcttctt gggggtgttt gtttctttct cggtttgctg gttggaagac gaagatgagg
      601 aggagctggt gctggccctc gaatcgtcat ccgacatatg gctgccgcgc ggcaccaggc
      661 cgctgctgtg atgatgatga tgatggctgc tgcccatggt atatctcctt cttaaagtta
      721 aacaaaatta tttctagagg ggaattgtta tccgctcaca attcccctat agtgagtcgt
      781 attaatttcg cgggatcgag atctcgatcc tctacgccgg acgcatcgtg gccggcatca
      841 ccggcgccca caggtgcggt tgctggcgcc tatatcg
//
