LOCUS       sequence-31222-e         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PPI40
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             74..91
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             634..651
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(704..722)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      718..765
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aaatattttg tttactttaa gaaggagata taccatgggc agcagccatc atcatcatca
       61 tcacagcagc ggcctggttc cgcgtggtag tcccattgct aaaaaggccc gctcaaatcc
      121 tcaggtgtac atggacatca agattgggaa caagccggct ggccgcatcc agatgctcct
      181 gcgttctgat gtcgtgccca tgacagcaga gaatttccgc tgcctgtgca ctcatgaaaa
      241 gggctttggc tttaagggaa gcagcttcca ccgcatcatc ccccagttca tgtgccaggg
      301 cggtgatttc acaaaccaca atggcactgg gggcaagtcc atctatggga agaagttcga
      361 tgatgaaaac tttatcctca agcatacggg accaggtcta ctatccatgg ccaactctgg
      421 cccaaacacc aatggctctc agttcttcct gacatgtgac aagacagact ggctggatgg
      481 caagcatgtg gtgtttggag aggtcaccga aggcctagat gtcttgcggc aaattgaggc
      541 ccagggcagc aaggacggga agccaaagca gaaggtgatc atcgccgact gtggggagta
      601 cgtgtgatga cgaagcttgc ggccgcactc gagcaccacc accaccacca ctgagatccg
      661 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
      721 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
      781 atatccggat tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
      841 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct ccttttcgct
      901 ttcttccctt cctttctcgc cacgttcg
//
