LOCUS       sequence-312258-        4371 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC26A11_STOP
FEATURES             Location/Qualifiers
     source          1..4371
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2496..2595)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2612..2631)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2613..2631)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2636..2652)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2636..2652)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2754..2773)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2765..3574
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2832..2851)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3721..4309
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4210..4229
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgccca gctccgtgac cgccctggga caggcacggt ctagcggacc
      721 aggaatggca ccaagcgcct gctgttgctc ccctgccgcc ctgcagcgga gactgccaat
      781 cctggcatgg ctgcctagct actccctgca gtggctgaag atggacttcg tggcaggact
      841 gtccgtggga ctgaccgcaa tccctcaggc cctggcatac gcagaggtgg caggactgcc
      901 acctcagtac ggcctgtata gcgccttcat gggctgtttc gtgtacttct tcctgggcac
      961 ctccagagat gtgaccctgg gccccacagc catcatgtcc ctgctggtgt ctttctacac
     1021 atttcacgag cctgcctatg ccgtgctgct ggcctttctg agcggatgca tccagctggc
     1081 aatgggcgtg ctgaggctgg gcttcctgct ggactttatc tcctacccag tgatcaaggg
     1141 ctttacctct gccgcagcag tgactatagg ttttgggcaa atcaagaatc tgctgggcct
     1201 gcagaatatc cccagacctt tctttctgca ggtgtatcac accttcctga ggatcgccga
     1261 gacaagggtg ggcgacgccg tgctgggact ggtgtgcatg ctgctgctgc tggtgctgaa
     1321 gctgatgcgg gatcacgtgc cacccgtgca cccagagatg cctccaggcg tgaggctgtc
     1381 caggggactg gtgtgggcag caaccacagc aagaaacgca ctggtggtgt ctttcgccgc
     1441 cctggtggcc tacagctttg aggtgaccgg ctatcagccc ttcatcctga ccggagagac
     1501 agcagaggga ctgccacctg tgcggatccc accctttagc gtgaccacag ccaatggcac
     1561 catctccttc acagagatgg tgcaggacat gggagcagga ctggcagtgg tgcccctgat
     1621 gggcctgctg gagtctatcg ccgtggccaa ggcctttgcc agccagaaca attacaggat
     1681 cgatgccaac caggagctgc tggccatcgg cctgaccaat atgctgggct ccctggtgtc
     1741 ctcttatcca gtgaccggct cttttggcag gacagcagtg aacgcacaga gcggcgtgtg
     1801 caccccagca ggaggactgg tgacaggcgt gctggtgctg ctgtctctgg attacctgac
     1861 aagcctgttc tactatatcc ctaagagcgc cctggcagcc gtgatcatca tggcagtggc
     1921 accactgttc gacaccaaga tctttaggac actgtggcgg gtgaagagac tggatctgct
     1981 gcccctgtgc gtgaccttcc tgctgtgctt ttgggaggtg cagtatggaa tcctggcagg
     2041 cgccctggtg agcctgctga tgctgctgca ctccgccgca aggcctgaga caaaggtgtc
     2101 tgagggacca gtgctggtgc tgcagccagc atctggactg agcttcccag caatggaggc
     2161 cctgagggag gagatcctgt ccagagccct ggaggtgtct cctccacgct gcctggtgct
     2221 ggagtgtacc cacgtgtgca gcatcgacta cacagtggtg ctgggactgg gagagctgct
     2281 gcaggatttt cagaagcagg gagtggccct ggccttcgtg ggactgcagg tgcctgtgct
     2341 gcgcgtgctg ctgtccgccg acctgaaggg cttccagtac ttttctaccc tggaggaggc
     2401 cgagaagcac ctgaggcagg agcctggcac acagccatat aatatccgcg aggactccat
     2461 cctggatcag aaggtggccc tgctgaaggc ctaagaccca gctttcttgt acaaagttgg
     2521 cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa
     2581 taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat
     2641 agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga
     2701 taaaataata tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg
     2761 tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc
     2821 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta
     2881 tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt
     2941 tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct
     3001 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat
     3061 ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt
     3121 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt
     3181 taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt
     3241 tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga
     3301 aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact
     3361 tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg
     3421 aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc
     3481 ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt
     3541 gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca
     3601 ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa
     3661 cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3721 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3781 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3841 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     3901 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3961 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     4021 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     4081 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     4141 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     4201 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     4261 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     4321 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt t
//
