LOCUS       sequence-31226-e         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: OTUB1
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             74..91
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             817..834
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(887..905)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 aaatattttg tttactttaa gaaggagata taccatgggc agcagccatc atcatcatca
       61 tcacagcagc ggcctggttc cgcgtggtag tgagattgct gtgcagaacc ctctggtgtc
      121 agagcggctg gagctctcgg tcctatacaa ggagtatgct gaagatgaca acatctatca
      181 acagaagatc aaggacctcc acaaaaagta ctcgtacatc cgcaagacca ggcctgacgg
      241 caactgtttc tatcgggctt tcggattctc ccacttggag gcactgctgg atgacagcaa
      301 ggagttgcag cggttcaagg ctgtgtctgc caagagcaag gaagacctgg tgtcccaggg
      361 cttcactgaa ttcacaattg aggatttcca caacacgttc atggacctga ttgagcaggt
      421 ggagaagcag acctctgtcg ccgacctgct ggcctccttc aatgaccaga gcacctccga
      481 ctaccttgtg gtctacctgc ggctgctcac ctcgggctac ctgcagcgcg agagcaagtt
      541 cttcgagcac ttcatcgagg gtggacggac tgtcaaggag ttctgccagc aggaggtgga
      601 gcccatgtgc aaggagagcg accacatcca catcattgcg ctggcccagg ccctcagcgt
      661 gtccatccag gtggagtaca tggaccgcgg cgagggcggc accaccaatc cgcacatctt
      721 ccctgagggc tccgagccca aggtctacct tctctaccgg cctggacact acgatatcct
      781 ctacaaatga tgacgaagct tgcggccgca ctcgagcacc accaccacca ccactgagat
      841 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
      901 ctagcataac cccttggggc ctct
//
