LOCUS       sequence-31227-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PPPDE1
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..92
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             626..643
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      711..758
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tgaatatttt gtttacttta agaaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcctggtt ccgcgtggta gtatggagcc gccgaatctc tatccggtga
      121 agctctacgt gtacgacctg tccaaaggcc tggcccggcg gctcagcccc atcatgctgg
      181 ggaaacaact ggaaggcatc tggcacacat ccatagttgt gcacaaggat gagttcttct
      241 tcggcagtgg tggtatctcc agctgccccc cgggagggac attgcttggg cctccagact
      301 ctgtggttga tgtggggagt acagaagtca cagaagaaat ctttctggag tacctctcct
      361 ccctggggga gtccctgttc cgaggtgagg cctacaacct ctttgaacac aattgtaaca
      421 ccttcagcaa cgaagtggca cagttcctga ctgggcggaa gattccttct tacatcacag
      481 acctgccctc tgaagttctc tccacgccct ttggacaggc acttcggccc ctcctggact
      541 ccattcagat ccagcctcca ggagggagct ccgtgggcag acccaacggc cagagctgat
      601 gacgaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa
      661 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaaataa ctagcataac
      721 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
      781 gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
      841 gcgcagcgtg accgctacac ttg
//
