LOCUS       sequence-31228-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PLAP
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..92
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ctgaatattt tgtttacttt agaaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcctggtt ccgcgtggta gtatggccgg agttgatcca tttacaggga
      121 atagtgccta ccgatcagct gcatctaaaa caatgaatat ttatttccct aaaaaagagg
      181 ctgtcacatt tgaccaagca aaccctacac aaatattagg taaactgaag gaacttaatg
      241 gaactgcacc tgaagagaag aagttaactg aggatgactt gatacttctt gagaagatac
      301 tgtctctaat atgtaatagt tcttcagaaa aacccacaat ccagcaactt cagattttgt
      361 ggaaagctat taactgtcct gaagatattg tctttcctgc acttgacatt cttcggttgt
      421 caattaaaca ccccagtgtg aatgagaact tctgcaatga aaaggaaggg gctcagttca
      481 gcagtcatct tatcaatctt ctgaacccta aaggaaagcc agcaaaccag ctgcttgctc
      541 tcaggacttt ttgcaattgt tttgttggcc aggcaggaca aaaactcatg atgtcccaga
      601 gggaatcact gatgtcccat gcaatagaac tgaaatcagg gagcaataag aacattcaca
      661 ttgctctggc tacattggcc ctgaactatt ctgtttgttt tcataaagac cataacattg
      721 aagggaaagc ccaatgtttg tcactaatta gcacaatctt ggaagtagta caagacctag
      781 aagccacttt tagacttctt gtggctcttg gaacacttat cagtgatgat tcaaatgctg
      841 tacaattagc
//
