LOCUS       sequence-312282-        4770 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4770)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC26A2_STOP
FEATURES             Location/Qualifiers
     source          1..4770
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2895..2994)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3011..3030)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3012..3030)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3035..3051)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3035..3051)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3153..3172)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3164..3973
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3231..3250)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4120..4708
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4609..4628
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgagct ccgagtccaa ggagcagcac aacgtgagcc ctagggactc
      721 cgccgagggc aatgatagct atccatccgg catccacctg gagctgcagc gcgagtctag
      781 caccgacttc aagcagtttg agacaaacga tcagtgccgg ccttaccaca gaatcctgat
      841 tgagagacag gagaagtccg acaccaattt caaggagttt gtgatcaaga agctgcagaa
      901 gaactgccag tgttctccag ccaaggccaa gaatatgatc ctgggcttcc tgcctgtgct
      961 gcagtggctg ccaaagtatg acctgaagaa gaacatcctg ggcgatgtga tgagcggcct
     1021 gatcgtgggc atcctgctgg tgccccagtc tatcgcatat agcctgctgg caggacagga
     1081 gcccgtgtac ggcctgtata cctccttctt tgcctctatc atctacttcc tgctgggcac
     1141 aagccggcac atctccgtgg gcatctttgg tgtgttgtgt ttgatgatag gcgagaccgt
     1201 ggacagagag ctgcagaagg ccggctacga taatgcacac tctgccccaa gcctgggaat
     1261 ggtgtccaac ggatctaccc tgctgaatca cacatccgac aggatctgcg ataagtcttg
     1321 ttacgccatc atggtgggct ctaccgtgac attcatcgcc ggcgtgtatc aggtggccat
     1381 gggcttcttt caagtgggct tcgtgagcgt gtacctgagc gacgcactgc tgagcggatt
     1441 cgtgaccgga gcctctttta ccatcctgac aagccaggcc aagtatctgc tgggcctgaa
     1501 cctgccaagg acaaatggcg tgggctccct gatcaccaca tggatccacg tgttccgcaa
     1561 catccacaag accaatctgt gcgatctgat cacaagcctg ctgtgcctgc tggtgctgct
     1621 gcccaccaag gagctgaacg agcactttaa gtccaagctg aaggccccaa tcccaatcga
     1681 gctggtggtg gtggtggcag ccaccctggc ctcccacttc ggcaagctgc acgagaacta
     1741 caattcctct atcgccggcc acatccccac aggctttatg ccccctaagg tgcccgagtg
     1801 gaatctgatc ccttccgtgg ccgtggatgc catcgccatc tctatcatcg gcttcgccat
     1861 caccgtgagc ctgtccgaga tgtttgccaa gaagcacggc tacacagtga aggccaacca
     1921 ggagatgtat gccatcggct tctgcaatat catccctagc ttctttcact gttttaccac
     1981 atccgccgcc ctggcaaaga ccctggtgaa ggagtctacc ggatgccaca cacagctgag
     2041 cggagtggtg acagccctgg tgctgctgct ggtgctgctg gtcatcgccc cactgttcta
     2101 ctccctgcag aagtctgtgc tgggcgtgat caccatcgtg aacctgaggg gcgccctgag
     2161 gaagttccgc gacctgccca agatgtggtc tatcagcaga atggataccg tgatctggtt
     2221 tgtgacaatg ctgagctccg ccctgctgtc taccgagatc ggcctgctgg tgggcgtgtg
     2281 cttcagcatc ttctgcgtga tcctgaggac acagaagccc aagtctagcc tgctgggcct
     2341 ggtggaggag tctgaggtgt tcgagtccgt gtctgcctat aagaatctgc agatcaagcc
     2401 aggcatcaag atcttccgct ttgtggcccc cctgtactat atcaacaagg agtgctttaa
     2461 gagcgccctg tacaagcaga ccgtgaatcc catcctgatc aaggtggcct ggaagaaggc
     2521 cgccaagcgg aagatcaagg agaaggtggt gaccctgggc ggcatccagg acgagatgag
     2581 cgtgcagctg tcccacgatc ctctggagct gcacacaatc gtgatcgact gttccgccat
     2641 ccagttcctg gataccgccg gcatccacac actgaaggag gtgcggagag actatgaggc
     2701 catcggcatc caggtgctgc tggcccagtg caaccctacc gtgagagata gcctgacaaa
     2761 tggcgagtac tgtaagaagg aggaggagaa cctgctgttc tacagcgtgt atgaggccat
     2821 ggcctttgcc gaggtgtcca agaaccagaa gggcgtgtgc gtgccaaatg gcctgagcct
     2881 gtcctctgac taagacccag ctttcttgta caaagttggc attataagaa agcattgctt
     2941 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc
     3001 agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg
     3061 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaataatat catcatgaac
     3121 aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg
     3181 ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg
     3241 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga
     3301 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga
     3361 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat
     3421 ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca
     3481 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct
     3541 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg
     3601 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga
     3661 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt
     3721 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga
     3781 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga
     3841 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt
     3901 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga
     3961 tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac
     4021 ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca
     4081 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     4141 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     4201 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     4261 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     4321 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     4381 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4441 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     4501 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4561 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     4621 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     4681 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     4741 ggccttttgc tggccttttg ctcacatgtt
//
