LOCUS       sequence-31229-e         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: KRASB
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             626..643
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(696..714)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      710..757
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tgaatatttt gtttacttta agaaggagat ataccatgca tcatcatcat catcacagca
       61 gcggcagaga aaacttgtat ttccagggca tgactgaata taaacttgtg gtagttggag
      121 ctggtggcgt aggcaagagt gccttgacga tacagctaat tcagaatcat tttgtggacg
      181 aatatgatcc aacaatagag gattcctaca ggaagcaagt agtaattgat ggagaaacct
      241 gtctcttgga tattctcgac acagcaggtc atgaggagta cagtgcaatg agggaccagt
      301 acatgaggac tggggagggc tttctttgtg tatttgccat aaataatact aaatcatttg
      361 aagatattca ccattataga gaacaaatta aaagagttaa ggactctgaa gatgtaccta
      421 tggtcctagt aggaaataaa tgtgatttgc cttctagaac agtagacaca aaacaggctc
      481 aggacttagc aagaagttat ggaattcctt ttattgaaac atcagcaaag acaagacagg
      541 gtgttgatga tgccttctat acattagttc gagaaattcg aaaacataaa gaaaagtgat
      601 gacgaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa
      661 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc
      721 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg
      781 attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
      841 cgcagcgtga ccgctacact ttgccagcgc cctagcgccc gctcctttcg cttcctnccc
      901 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ct
//
