LOCUS       sequence-31231-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MBD4
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             76..96
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             576..593
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(646..664)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      660..707
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(831..850)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttccctctga atattttgtt tactttaaga aggagatata ccatgcatca tcatcatcat
       61 cacagcagcg gcagagaaaa cttgtatttc cagggccgac gtaaagcctt taagaaatgg
      121 acacctcctc ggtcaccttt taatctcgtt caagaaacac tttttcatga tccatggaag
      181 cttctcatcg ctactatatt tctcaatcgg acctcaggca aaatggcaat acctgtgctt
      241 tggaagtttc tggagaagta tccttcagct gaggtagcaa gaaccgcaga ctggagagat
      301 gtgtcagaac ttcttaaacc tcttggtctc tacgatcttc gggcaaaaac cattgtcaag
      361 ttctcagatg aatacctgac aaagcagtgg aagtatccaa ttgagcttca tgggattggt
      421 aaatatggca acgactctta ccgaattttt tgtgtcaatg agtggaagca ggtgcaccct
      481 gaagaccaca aattaaataa atatcatgac tggctttggg aaaatcatga aaaattaagt
      541 ctatcttgat gacgaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc
      601 cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac
      661 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa
      721 ctatatccgg attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
      781 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
      841 tttcttccct tcctttctcg c
//
