LOCUS       sequence-31232-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ASMTL
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..88
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             808..825
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(878..896)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 attttgttta actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca
       61 cagcagcggc ctggttccgc gtggtagtct gctgcacaag cgcgtggtgc tggccagcgc
      121 ctccccacgc cgtcaggaga tcctcagcaa cgcgggtctc aggtttgagg tggtcccctc
      181 caagtttaaa gagaagctgg acaaagcctc cttcgctact ccgtatgggt acgccatgga
      241 gaccgccaag cagaaggccc tggaggtggc caaccggctg taccagaaag acctgcgggc
      301 ccccgacgtg gtcattggag cggacacgat cgtgacggtc ggggggctga ttctggagaa
      361 gccggtggac aagcaggacg cctacaggat gctgtcccgg ttgagtggga gagaacacag
      421 cgtgttcaca ggtgtcgcga tcgtccactg ctccagcaaa gaccatcagc tggacaccag
      481 ggtctcggaa ttctacgagg aaacgaaggt gaagttctcg gagctgtccg aggagctgct
      541 ctgggaatac gtccacagcg gggagcccat ggacaaagct ggcggctacg ggatccaggc
      601 cctgggcggc atgctggtgg agtccgtaca cggggacttt ctgaacgtgg tgggattccc
      661 gctgaaccac ttctgcaagc agctggtgaa gctctactac ccgccccgcc cggaggacct
      721 gcggcggagt gtcaagcacg actccatccc ggccgcggac accttcgaag acctcagttg
      781 atgacgaagc ttgcggccgc actcgagcac caccaccacc accactgaga tccggctgct
      841 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcat
//
