LOCUS       sequence-312340-        4383 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC23A3_STOP
FEATURES             Location/Qualifiers
     source          1..4383
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2508..2607)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2624..2643)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2625..2643)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2648..2664)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2648..2664)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2766..2785)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2777..3586
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2844..2863)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3733..4321
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4222..4241
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgtccc ggtctcccct gaacccttcc cagctgagat ctgtgggcag
      721 ccaggacgca ctggcaccac tgccacctcc agcaccacag aatccctcca cccactcttg
      781 ggatccactg tgcggaagcc tgccatgggg actgtcctgt ctgctggccc tgcagcacgt
      841 gctggtcatg gccagcctgc tgtgcgtgtc ccacctgctg ctgctgtgca gcctgtcccc
      901 aggaggactg tcttacagcc cttcccagct gctggccagc tccttcttta gctgcggcat
      961 gtccaccatc ctgcagacat ggatgggatc caggctgcca ctggtgcagg caccttctct
     1021 ggagttcctg atccctgccc tggtgctgac ctcccagaag ctgcctcggg ccatccagac
     1081 accaggcaac tctagcctga tgctgcacct gtgcagagga ccaagctgtc acggactggg
     1141 acactggaat acatctctgc aggaggtgag cggagcagtg gtggtgtctg gactgctgca
     1201 gggaatgatg ggactgctgg gaagccctgg acacgtgttt ccacactgcg gacctctggt
     1261 gctggcacca agcctggtgg tggcaggact gtccgcccac agggaggtgg cacagttctg
     1321 ctttacccac tggggactgg ccctgctggt catcctgctg atggtggtgt gctcccagca
     1381 cctgggctct tgccagttcc acgtgtgccc ctggcggaga gcctctacat cctctaccca
     1441 cacaccactg cccgtgttta ggctgctgag cgtgctgatc ccagtggcct gcgtgtggat
     1501 cgtgtctgcc ttcgtgggct ttagcgtgat cccacaggag ctgagcgccc caaccaaggc
     1561 accatggatc tggctgcctc acccaggcga gtggaactgg cctctgctga ccccacgcgc
     1621 cctggcagca ggaatctcca tggcactggc agcatctaca agctccctgg gatgctatgc
     1681 actgtgcggc aggctgctgc acctgccacc tccaccccct cacgcatgct ctaggggact
     1741 gagcctggag ggactgggat ctgtgctggc aggcctgctg ggcagcccta tgggcaccgc
     1801 ctctagcttc ccaaacgtgg gcaaggtcgg actgatccag gcaggatccc agcaggtggc
     1861 acacctggtg ggcctgctgt gcgtgggact gggactgagc ccaaggctgg cccagctgct
     1921 gaccacaatc ccactgcctg tggtgggagg cgtgctggga gtgacacagg cagtggtgct
     1981 gtccgccggc ttctcctctt tttacctggc cgacatcgat agcggcagaa atatctttat
     2041 cgtgggcttc tccatcttta tggccctgct gctgccaagg tggttcaggg aggcacccgt
     2101 gctgttttcc accggctggt ctccactgga cgtgctgctg cactccctgc tgacacagcc
     2161 catcttcctg gccggcctgt ctggctttct gctggagaac accatccctg gaacacagct
     2221 ggagagggga ctgggacagg gactgccaag ccccttcacc gcacaggagg caaggatgcc
     2281 tcagaagcca cgcgagaagg ccgcccaggt gtatcgcctg ccttttccaa tccagaatct
     2341 gtgcccctgt atcccccagc ctctgcactg cctgtgccca ctgccagagg accctggcga
     2401 tgaggaggga ggaagctccg agccagagga gatggcagat ctgctgcctg gaagcggaga
     2461 gccttgccca gagtctagca gggagggctt cagatcccag aagtaagacc cagctttctt
     2521 gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac
     2581 tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta
     2641 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
     2701 cattgcacaa gataaaataa tatcatcatg aacaataaaa ctgtctgctt acataaacag
     2761 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc
     2821 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
     2881 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     2941 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
     3001 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     3061 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     3121 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     3181 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     3241 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     3301 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
     3361 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
     3421 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
     3481 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
     3541 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
     3601 ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc
     3661 aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat
     3721 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3781 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3841 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     3901 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3961 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     4021 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     4081 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     4141 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     4201 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     4261 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     4321 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     4381 gtt
//
