LOCUS       sequence-31235-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CENTG3 (3IHW)
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             76..96
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             624..641
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(694..712)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      708..754
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attcctctga atattttgtt tactttaaga aggagatata ccatgcatca tcatcatcat
       61 cacagcagcg gcagagaaaa cttgtatttc cagggcccgg aactgaaagt tggcatcgtc
      121 ggcaacctgt cgagcggcaa gtctgcgctg gtacatcgct atctgaccgg tacttacgtt
      181 caagaggaaa gtccggaggg gggtcgtttc aagaaagaga ttgttgtcga cggccagtca
      241 tatctgctgc tgatccgtga cgaaggtggg ccgccggaac tgcagttcgc tgcgtgggtg
      301 gacgccgtag ttttcgtgtt ttccctggag gacgaaattt cgttccagac agtctacaat
      361 tattttctgc gtctgtgtag ctttcgcaac gcgtctgagg ttccgatggt actggtcggg
      421 acgcaagacg ccatcagtgc cgcaaatccg cgtgtcattg acgattcacg cgcgcgtaaa
      481 ctgtcaacag atctgaaacg ttgcacgtat tacgagacat gtgctaccta cgggctgaac
      541 gttgagcgtg tgtttcagga cgtagcccaa aaagtcgttg cgctgcgtaa aaagtgatga
      601 cgaagcttgc ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaaca
      661 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc
      721 ttggggcctc taaacgggtc ttgagggggt tttttgctga aaggaggaac tatatccgga
      781 ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgt
//
