LOCUS       sequence-31238-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CYP51A1 (3I3K)
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(169..174,175..192,193..199)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    207..223
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        join(278..283,284..299,300..306)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    314..330
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        join(373..378,379..394,395..401)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    409..425
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ttcattccca ccctgaattg actctcttcc gggcgcctat catgccatac cgcgaaaggt
       61 tttgcaccat tcgatggtgt cctggcacga caggtttccc gactggaaag cgggcagtga
      121 gcgcaacgca attaatgtaa gttagctcac tcattaggca ccccaggctt tacactttat
      181 gcttccggct cgtataatgt gtggaattgt gagcggataa caatttcaca caggaaacag
      241 gatcgatcca tcgatgagct tactccccat ccccctgttg acaattaatc atcggctcgt
      301 ataatgtgtg gaattgtgag cggataacaa tttcacacag gaaacaggat cagcttactc
      361 cccatccccc tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat
      421 aacaatttca cacaggaaac aggatccatc gatgcttagg aggtcatatg gctaagaaaa
      481 cgctgcccgc aggggtgaaa agtcctccat acattttctc cccaattcca ttccttgggc
      541 atgccatagc atttgggaaa agtccaattg aatttctaga aaatgcatat gagaagtatg
      601 gacctgtatt tagttttacc atggtaggca agacatttac ttaccttctg gggagtgatg
      661 ctgctgcact gctttttaat agtaaaaatg aagacctgaa tgcagaagat gtctacagtc
      721 gcctgacaac acctgtgttt gggaagggag ttgcatacga tgtgcctaat ccagttttct
      781 tggagcagaa gaaaatgtta aaaagtggcc ttaacatagc ccactttaaa cagcatgttt
      841 ctat
//
