LOCUS       sequence-31239-e         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DMAP
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..90
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             396..413
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(466..484)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      480..527
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(651..670)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     862..883
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ctgaatattt tgtttacttt aagaaggaga tataccatgc atcatcatca tcatcacagc
       61 agcggcagag aaaacttgta tttccagggc ggcaaggact acccctttgc caggttcaat
      121 aagactgtgc aggtgcctgt gtactcggag caggagtacc agctttatct ccacgatgat
      181 gcttggacta aggcagaaac tgaccacctc tttgacctca gccgccgctt tgacctgcgt
      241 tttgttgtta tccatgaccg gtatgaccac cagcagttca agaagcgttc tgtggaagac
      301 ctgaaggagc ggtactacca catctgtgct aagcttgcca acgtgcgggc tgtgccaggc
      361 acagactgat gacgaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc
      421 cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac
      481 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa
      541 ctatatccgg attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
      601 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
      661 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
      721 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
      781 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
      841 tggagtccac gttctttaat agtggactct tgttccaaac tggaac
//
