LOCUS       sequence-31240-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DNMT3B
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             67..87
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             567..584
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(637..655)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      651..698
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(822..841)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aatattttgt ttactttaag aaggagatat accatgcatc atcatcatca tcacagcagc
       61 ggcagagaaa acttgtattt ccagggcgag gcagacagtg gagatggaga cagttcagag
      121 tatcaggatg ggaaggagtt tggaataggg gacctcgtgt ggggaaagat caagggcttc
      181 tcctggtggc ccgccatggt ggtgtcttgg aaggctacct ctaagcgtca ggccatgagt
      241 ggcatgcgct gggtccaatg gtttggtgac gggaaattct cagaagtatc cgcagacaaa
      301 ctggtggctc tgggactgtt ctcgcagcat ttcaatctgg cgacgtttaa caagctggta
      361 tcttatcgta aagccatgta ccacgcactg gagaaagctc gcgtccgtgc gggtaagact
      421 ttcccgtcta gtccgggtga cagcctggag gaccagctga agcctatgct ggagtgggct
      481 cacgggggtt ttaaacctac cggaatcgag gggctgaagc cgaataacac ccagccttga
      541 tgacgaagct tgcggccgca ctcgagcacc accaccacca ccactgagat ccggctgcta
      601 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
      661 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
      721 gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
      781 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
      841 ttcctttctc gccacgttcg ccggc
//
