LOCUS       sequence-31241-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: UBH12 (3DB3)
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             66..86
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             596..613
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(666..684)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      680..727
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 atattttgtt tactttaaga aggagatata ccatgcatca tcatcatcat cacagcagcg
       61 gcagagaaaa cttgtatttc cagggcatgt gggatgagac ggaattgggg ctgtacaagg
      121 tcaatgagta cgtcgatgct cgggacacga acatgggggc gtggtttgag gcgcaggtgg
      181 tcagggtgac gcggaaggcc ccctcccggg acgagccctg cagctccacg tccaggccgg
      241 cgctggagga ggacgtcatt taccacgtga aatacgacga ctacccggag aacggcgtgg
      301 tccagatgaa ctccagggac gtccgagcgc gcgcccgcac catcatcaag tggcaggacc
      361 tggaggtggg ccaggtggtc atgctcaact acaaccccga caaccccaag gagcggggct
      421 tctggtacga cgcggagatc tccaggaagc gcgagaccag gacggcgcgg gaactctacg
      481 ccaacgtggt gctgggggat gattctctga acgactgtcg gatcatcttc gtggacgaag
      541 tcttcaagat tgagcggccg ggtgaatgat gacgaagctt gcggccgcac tcgagcacca
      601 ccaccaccac cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc
      661 tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg
      721 ttttttgctg aaaggaggaa ctatatccgg attggcgaat gggacgcgcc ctgtagcggc
      781 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
      841 ctagcgcccg ct
//
