LOCUS       sequence-31244-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FKBP06
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             41..58
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..91
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             520..537
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(590..608)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      604..651
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tctgaatatt ttgtttactt taagaaggag atataccatg catcatcatc atcatcacag
       61 cagcggcaga gaaaacttgt atttccaggg cgaaggggac gacgcccccg gccagtccct
      121 gtacgagcgg ttaagtcaga ggatgctgga catctcgggg gaccggggcg tgctgaagga
      181 cgtcatccga gaaggagctg gagacctagt ggcgcctgat gcttcggtgc tagtgaaata
      241 ctcgggatac ctggaacaca tggacagacc cttcgattct aattacttta ggaaaactcc
      301 tcggctaatg aaacttggag aggatattac actgtggggc atggagctgg gccttctgag
      361 catgcggaga ggagagctgg ccaggtttct gttcaaaccg aactacgcct atggaacgct
      421 gggctgccct cccttgatcc ccccaaacac cactgtcctg tttgagattg agctgcttga
      481 cttcctggac tgatgacgaa gcttgcggcc gcactcgagc accaccacca ccaccactga
      541 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
      601 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga
      661 ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
      721 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
      781 tcgcttttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
      841 cgggggctcc ctttagggtt ccgatttagt gctttacggc accctcga
//
