LOCUS       sequence-31247-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NEDD4L
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             67..87
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gaatattttg tttactttag aaggagatat accatgcatc atcatcatca tcacagcagc
       61 ggcagagaaa acttgtattt ccagggctcc agagaattta agcagaaata tgactacttc
      121 aggaagaaat taaagaaacc tgctgatatc cccaataggt ttgaaatgaa acttcacaga
      181 aataacatat ttgaagagtc ctatcggaga attatgtccg tgaaaagacc agatgtccta
      241 aaagctagac tgtggattga gtttgaatca gagaaaggtc ttgactatgg gggtgtggcc
      301 agagaatggt tcttcttact gtccaaagag atgttcaacc cctactacgg cctctttgag
      361 tactctgcca cggacaacta cacccttcag atcaacccta attcaggcct ctgtaatgag
      421 gatcatttgt cctacttcac ttttattgga agagttgctg gtctggccgt atttcatggg
      481 aagctcttag atggtttctt cattagacca ttttacaaga tgatgttggg aaagcagata
      541 accctgaatg acatggaatc tgtggatagt gaatattaca actctttgaa atggatcctg
      601 gagaatgacc ctactgagct ggacctcatg ttctgcatag acgaagaaaa ctttggacag
      661 acatatcaag tggatttgaa gcccaatggg tcagaaataa tggtcacaaa tgaaaacaaa
      721 agggaatata tcgacttagt catccagtgg agatttgtga acagggtcca gaagcagatg
      781 aacgccttct tggagggatt cacagaacta cttcctattg atttgattaa aatttttgat
      841 gaaaatgagc tggagttgct catgtgcggc ctcggtgatg tggatgtg
//
