LOCUS       sequence-31249-e         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: UBH12 (3DB4)
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..90
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             732..749
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(802..820)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      816..863
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 atattttgtt tanctttaag aaggagatat accatgggca gcagccatca tcatcatcat
       61 cacagcagcg gcctggttcc gcgtggtagt ccgtccaacc actacggacc catcccgggg
      121 atccccgtgg gcaccatgtg gcggttccga gtccaggtca gcgagtcggg tgtccatcgg
      181 ccccacgtgg ctggcataca cggccggagc aacgacggag cgtactccct agtcctggcg
      241 gggggctatg aggatgacgt ggaccatggg aattttttca catacacggg tagtggtggt
      301 cgagatcttt ccggcaacaa gaggaccgcg gaacagtctt gtgatcagaa actcaccaac
      361 accaacaggg cgctggctct caactgcttt gctcccatca atgaccaaga aggggccgag
      421 gccaaggact ggcggtcggg gaagccggtc agggtggtgc gcaatgtcaa gggtggcaag
      481 aatagcaagt acgcccccgc tgagggcaac cgctatgatg gcatctacaa ggttgtgaaa
      541 tactggcccg agaaggggaa gtccgggttt ctcgtgtggc gctaccttct gcggagggac
      601 gatgatgagc ctggccattg gacgaaggag gggaaggacc ggatcaagaa gctggggctg
      661 accatgcagt atccagaagg ctacctggaa gccctggcca actgatgacg aagcttgcgg
      721 ccgcactcga gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg
      781 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
      841 aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggattg gcgaatggga
      901 cgcgcccctg tagcggcgca ttaagcgcgg c
//
