LOCUS       sequence-31251-e         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IQGAP3
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..55
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..88
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             427..444
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(497..515)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      511..558
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(682..701)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tgaatatttt gtttacttta gaaggagata taccatgcat catcatcatc atcacagcag
       61 cggcagagaa aacttgtatt tccagggcgg caaaaaacag ccgagcctgc attatacagc
      121 cgcgcagctg ctggagaagg gcgtgctggt tgaaattgag gaccttccgg ccagtcattt
      181 tcgtaatgtt atctttgata tcaccccggg ggacgaggcg gggaaatttg aagttaatgc
      241 caaattcctg ggtgtcgata tggagcgttt tcaactgcac taccaggatc tgctgcaact
      301 gcagtatgaa ggtgttgcgg tgatgaagct gttcaacaaa gccaaggtaa acgttaacct
      361 tctgatcttt ctgctgaata aaaagtttct gcgcaaatga tgacgaagct tgcggccgca
      421 ctcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct
      481 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
      541 gtcttgaggg gttttttgct gaaaggagga actatatccg gattggcgaa tgggacgcgc
      601 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
      661 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
      721 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
      781 tacggcacct cgaccccaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
      841 ctgatagacg gtttttcgcc cctttgacgt tggagtccac gttctttaat agtggactct
      901 tgttc
//
