LOCUS       sequence-31252-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ITSN2 (3GF9)
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..90
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tgaaatattt tgtttacttt aagaaggaga tataccatgc atcatcatca tcatcacagc
       61 agcggcagag aaaacttgta tttccagggc tgtcaggtga ttgctatgta tgactatgca
      121 gcaaataatg aagatgagct cagtttctcc aagggacaac tcattaatgt tatgaacaaa
      181 gatgatcctg attggtggca aggagagatc aacggggtga ctggtctctt tccttcaaac
      241 tacgttaaga tgacgacaga ctcagatcca agtcaacagt ggtgtgctga tctgcaaacc
      301 ctggacacaa tgcagccaat tgagaggaaa agacagggct atattcatga gctgattcag
      361 accgaagagc ggtacatggc tgaccttcag ctcgtcgtcg aggtttttca gaaacgcatg
      421 gcagagtcag gctttctcac tgaaggggag atggccctga tttttgttaa ctggaaggag
      481 ctcatcatgt ccaacacaaa gctgctgaag gctttgcggg tgcggaagaa gaccgggggc
      541 gagaagatgc cggtgcagat gattggggac atcctggccg ctgagctgtc ccacatgcag
      601 gcttacatca ggttctgcag ctgccagctt aatggagcag ctctgttaca gcagaagaca
      661 gatgaagaca cagatttcaa agaattttta aagaagctgg catctgaccc gcggtgtaaa
      721 ggaatgcccc tctccagctt cctgctgaaa cccatgcaga ggatcacccg ctacccactg
      781 ctcatcagaa gtattctgga gaacaccccg gagagccatg cagaccattc ctccctaaag
      841 ctggccctcg agcgg
//
