LOCUS       sequence-31253-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ITSN2 (3JZY)
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tgaaatattt tgtttacttt agaaggagat ataccatgca tcatcatcat catcacagca
       61 gcggcagaga aaacttgtat ttccagggcg acacaatgca gccaattgag aggaaaagac
      121 agggctatat tcatgagctg attcagaccg aagagcggta catggctgac cttcagctcg
      181 tcgtcgaggt ttttcagaaa cgcatggcag agtcaggctt tctcactgaa ggggagatgg
      241 ccctgatttt tgttaactgg aaggagctca tcatgtccaa cacaaagctg ctgaaggctt
      301 tgcgggtgcg gaagaagacc gggggcgaga agatgccggt gcagatgatt ggggacatcc
      361 tggccgctga gctgtcccac atgcaggctt acatcaggtt ctgcagctgc cagcttaatg
      421 gagcagctct gttacagcag aagacagatg aagacacaga tttcaaagaa tttttaaaga
      481 agctggcatc tgacccgcgg tgtaaaggaa tgcccctctc cagcttcctg ctgaaaccca
      541 tgcagaggat cacccgctac ccactgctca tcagaagtat tctggagaac accccggaga
      601 gccatgcaga ccattcctcc ctaaagctgg ccctcgagcg ggcagaggag ctgtgctctc
      661 aagtgaatga gggagttcgg gagaaggaaa actcggaccg actggagtgg atccaggcgc
      721 acgtgcagtg tgaaggcctc gcggagcaac ttattttcaa ctctctcacc aactgcctgg
      781 ggccccggaa gctcttacac agtgggaaat tatacaagac caagagcaac
//
