LOCUS       sequence-31255-e         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: LMNB1 (3HN9)
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..95
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             494..511
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(564..582)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      578..625
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(749..768)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tccctctgaa tattttgttt actttaagaa ggagatatac catgcatcat catcatcatc
       61 acagcagcgg cagagaaaac ttgtatttcc agggcagtag tgttagcatc tctcattccg
      121 cctcagccac tggaaatgtt tgcatcgaag aaattgatgt tgatgggaaa tttatccgct
      181 tgaagaacac ttctgaacag gatcaaccaa tgggaggctg ggagatgatc agaaaaattg
      241 gagacacatc agtcagttat aaatatacct caagatatgt gctgaaggca ggccagactg
      301 ttacaatttg ggctgcaaac gctggtgtca cagccagccc cccaactgac ctcatctgga
      361 agaaccagaa ctcgtggggc actggcgaag atgtgaaggt tatattgaaa aattctcagg
      421 gagaggaggt tgctcaaaga agtacagtct ttaaaacaac catatgatga cgaagcttgc
      481 ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa
      541 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
      601 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat tggcgaatgg
      661 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
      721 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      781 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      841 agtgctttac ggcaccctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      901 gccatcgccc tgatag
//
