LOCUS       sequence-31257-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MOCS3
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             72..89
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             497..514
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(567..585)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      581..628
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(752..771)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aatattttgt ttactttaga aggagatata ccatgggcag cagccatcat catcatcatc
       61 acagcagcgg cctggttccg cgtggtagtc gtgtttctgt caccgactat aagcgactgc
      121 tggattctgg ggcattccac ctgttgctgg acgtcaggcc tcaggtggag gtggacattt
      181 gtcgtttgcc tcatgcccta cacatccctc tgaaacattt ggaacgcagg gatgcggaga
      241 gcctgaaact cttaaaagaa gcaatctggg aagagaagca gggcacacaa gaaggggctg
      301 ctgtccccat ttatgtgatt tgcaaactgg gaaatgactc acagaaagcc gtgaagatcc
      361 tccagtcctt atcagcagct caagagttag accctttaac agttcgggat gttgtggggg
      421 gcctcatggc ctgggctgcc aaaatcgatg gaacatttcc acagtactga tgacgaagct
      481 tgcggccgca ctcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg
      541 aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc
      601 ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gattggcgaa
      661 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
      721 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
      781 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggct
//
