LOCUS       sequence-312616-        4560 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC9A6_STOP
FEATURES             Location/Qualifiers
     source          1..4560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2685..2784)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2801..2820)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2802..2820)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2825..2841)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2825..2841)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2943..2962)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2954..3763
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3021..3040)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3910..4498
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4399..4418
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatggctc ggagaggctg gaggcgcgcc cctctgcgga gaggagtggg
      721 aagctcccct agggcaaggc gcctgatgag accactgtgg ctgctgctgg cagtgggcgt
      781 gttcgattgg gcaggagcat ctgacggagg aggaggagag gcccgggcca tggatgagga
      841 gatcgtgagc gagaagcagg ccgaggagtc ccacagacag gactctgcca atctgctgat
      901 cttcatcctg ctgctgacac tgaccatcct gaccatctgg ctgtttaagc accggagagc
      961 caggttcctg cacgagacag gcctggccat gatctacgga ctgctggtgg gactggtgct
     1021 gcgctatggc atccacgtgc catccgatgt gaacaatgtg accctgtctt gcgaggtgca
     1081 gtctagcccc accacactgc tggtgacatt cgaccctgag gtgttcttta atatcctgct
     1141 gccccctatc atcttttacg ccggctattc cctgaagagg cgccacttct ttcggaacct
     1201 gggctctatc ctggcctacg cctttctggg caccgccatc agctgcttcg tgatcggctc
     1261 catcatgtat ggctgcgtga ccctgatgaa ggtgaccggc cagctggccg gcgatttcta
     1321 ctttaccgac tgtctgctgt tcggcgccat cgtgtccgcc acagatccag tgaccgtgct
     1381 ggccatcttc cacgagctgc aggtggacgt ggagctgtat gccctgctgt ttggcgagag
     1441 cgtgctgaat gacgccgtgg ccatcgtgct gtcctctagc atcgtggcat accagccagc
     1501 aggcgataac tctcacacat ttgacgtgac cgccatgttc aagagcatcg gcatcttcct
     1561 gggcatcttt tctggcagct tcgccatggg agcagcaaca ggagtggtga ccgccctggt
     1621 gacaaagttt accaagctga gggagttcca gctgctggag acaggcctgt tctttctgat
     1681 gtcctggtct acctttctgc tggcagaggc atggggcttc acaggagtgg tggccgtgct
     1741 gttttgcggc atcacacagg cccactacac ctataacaat ctgagcacag agtcccagca
     1801 ccgcaccaag cagctgtttg agctgctgaa cttcctggcc gagaatttca tcttttccta
     1861 tatgggcctg acactgttca cctttcagaa ccacgtgttt aatcctacct tcgtggtggg
     1921 cgcctttgtg gccatcttcc tgggcagggc cgccaacatc tacccactga gcctgctgct
     1981 gaatctgggc cggagatcta agatcggcag caattttcag cacatgatga tgttcgcagg
     2041 actgagggga gcaatggcct tcgccctggc catcagggat acagccacct acgcccgcca
     2101 gatgatgttc tccaccacac tgctgatcgt gttctttacc gtgtgggtgt ttggaggagg
     2161 aaccacagca atgctgagct gcctgcacat ccgggtgggc gtggattccg accaggagca
     2221 cctgggagtg ccagagaacg agaggaggac cacaaaggca gagtccgcct ggctgtttag
     2281 aatgtggtac aacttcgacc acaattatct gaagccactg ctgacccact ctggaccacc
     2341 actgaccaca accctgccag catgctgtgg acctatcgca agatgtctga ccagccctca
     2401 ggcctatgag aaccaggagc agctgaagga cgatgactcc gatctgatcc tgaatgatgg
     2461 cgacatctct ctgacctacg gcgacagcac agtgaacacc gagccagcca catcctctgc
     2521 cccacggaga ttcatgggca acagctccga ggatgcactg gacagggagc tggcctttgg
     2581 cgatcacgag ctggtcatca ggggaaccag gctggtgctg ccaatggatg acagcgagcc
     2641 tccactgaac ctgctggaca atacaagaca cggccccgcc taagacccag ctttcttgta
     2701 caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat
     2761 cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta
     2821 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat
     2881 tgcacaagat aaaataatat catcatgaac aataaaactg tctgcttaca taaacagtaa
     2941 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa
     3001 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
     3061 gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
     3121 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
     3181 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
     3241 cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
     3301 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
     3361 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
     3421 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
     3481 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
     3541 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg
     3601 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
     3661 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
     3721 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg
     3781 gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa
     3841 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3901 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3961 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     4021 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     4081 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     4141 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     4201 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     4261 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     4321 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4381 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4441 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4501 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
