LOCUS       sequence-31262-e         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HECTD1
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             66..86
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             329..346
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(399..417)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      413..460
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(584..603)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     794..815
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aatattttgt ttactttaga aggagatata ccatgcatca tcatcatcat cacagcagcg
       61 gcagagaaaa cttgtatttc cagggcctga aatacatggt tccaggagct cgtgttacca
      121 gaggcctgga ttggaaatgg cgagatcagg atggcagccc acagggagaa ggcactgtca
      181 caggagaact acacaatggc tggattgatg tcacctggga tgctggtggc tcaaactctt
      241 accgtatggg cgcagaagga aaatttgacc tcaagcttgc accagggtac gaccctgatt
      301 gatgacgaag cttgcggccg cactcgagca ccaccaccac caccactgag atccggctgc
      361 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
      421 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc
      481 cggattggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
      541 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
      601 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
      661 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
      721 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
      781 acgttcttta atagtggact cttgttccaa actggaac
//
