LOCUS       sequence-31264-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NEDD5
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggttccgcg tggtagtgaa acacctggct atgttggatt tgcaaacctc
      121 cccaatcaag ttcaccgaaa atcagtgaaa aaaggttttg agttcacact gatggtggtc
      181 ggtgaatcag gtctaggaaa atcgactctc ataaacagcc tattcctaac tgatctgtac
      241 ccagaaagag tcatatctgg agcagcagaa aaaattgaaa gaactgtcca gattgaggct
      301 tcaactgttg aaattgaaga gcgaggggtc aagctacgcc tgacagtggt agatacccct
      361 ggctatggtg acgctatcaa ctgcagagat tgttttaaga caattatctc ctatattgat
      421 gagcaatttg agaggtacct gcatgacgag agcggcttga acaggcggca catcattgat
      481 aatagggtgc attgttgctt ttactttatt tcaccttttg gacatggact taagccctta
      541 gatgtggcgt ttatgaaggc aatacacaac aaggtgaata ttgtgcctgt cattgcaaaa
      601 gctgacactc tcaccctgaa ggaacgggag cggctgaaga aaaggattct ggatgaaatt
      661 gaagaacata acatcaaaat ctatcactta cctgatgcag aatcagatga agatgaagat
      721 tttaaagagc agactagact tctcaaggct agcatcccat tctctgtggt tggatccaat
      781 cagttgattg aagccaaagg aaagaaggtc agaggccgcc tctaccccgc gggtgttgtg
      841 gaagtggaga acccagagca caatgacttt ctgaagctga gaaccatgct catcacccac
      901 atgcaggatc tccaggaggt ga
//
