LOCUS       sequence-31265-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MAP1LC3A
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             90..452
                     /codon_start=1
                     /gene="MAP1LC3A"
                     /product="human LC3 protein"
                     /label=LC3A
                     /note="Atg8 family member found on autophagosomes"
                     /translation="MPSDRPFKQRRSFADRCKEVQQIRDQHPSKIPVIIERYKGEKQLP
                     VLDKTKFLVPDHVNMSELVKIIRRRLQLNPTQAFFLLVNQHSMVSVSTPIADIYEQEKD
                     EDGFLYMVYASQETFGF"
     RBS             140..148
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             482..499
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(552..570)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      566..613
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(737..756)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tgaatatttt gtttacttta agaaggagat ataccatgca tcatcatcat catcacagca
       61 gcggcagaga aaacttgtat ttccagggca tgccctcaga ccggcctttc aagcagcggc
      121 ggagcttcgc cgaccgctgt aaggaggtac agcagatccg cgaccagcac cccagcaaaa
      181 tcccggtgat catcgagcgc tacaagggtg agaagcagct gcccgtcctg gacaagacca
      241 agtttttggt cccggaccat gtcaacatga gcgagttggt caagatcatc cggcgccgcc
      301 tgcagctgaa ccccacgcag gccttcttcc tgctggtgaa ccagcacagc atggtgagtg
      361 tgtccacgcc catcgcggac atctacgagc aggagaaaga cgaggacggc ttcctctata
      421 tggtctacgc ctcccaggaa accttcggct tctgatgacg aagcttgcgg ccgcactcga
      481 gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt
      541 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
      601 gaggggtttt ttgctgaaag gaggaactat atccggattg gcgaatggga cgcgccctgt
      661 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
      721 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
      781 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgc
//
